A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect LegioneUla species in bronchoalveolar fluid specimens. All LegioneUla strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective study,
The Amplicor HBV Monitor Test for quantitative detection of serum hepatitis B virus (HBV) DNA has recently been introduced. This assay is based on PCR and a non-radioactive hybridization and detection system on microwell plates. Evaluation in a routine diagnostic laboratory showed excellent sensitivity and adequate reproducibility; however, a more automated format would be desirable. The Amplicor HBV Monitor Test is useful for recognizing those patients who might benefit from antiviral treatment and for evaluation of the efficacy of anti-hepatitis B virus treatment.
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