Recent researches speak about the utilization of biological systems especially enterobacteria to synthesize nanoparticles is a novel approach. The present study demonstrates an eco-friendly and low cost protocol for synthesis of silver nano particles using the cell free filtrate of Lactobacillus bulgaricus when supplied with aqueous silver ions. Characterization of silver nanoparticles was done by UV-Visible absorption spectroscopy shows maximum absorption at 420 nm, Transmission electron microscope (TEM) revealed the formation of spherical nanoparticles with size ranging between 40-50nm, X-Ray diffraction (XRD) pattern shows spectrum, which reveals the FCC structure of silver nanoparticles. Energy dispersive spectroscope (EDS) analysis was done using SEM instrument equipped with thermo EDS attachment shows the optical absorption peak at 3kev. Microbial nanoparticles showed very strong inhibitory action against Staphylococcus aureus, Staphylococcus albus, & Pseudomonas aeruginosa.
Morinda citrifolia commonly called as Noni belongs to Rubiaceae family was used for synthesis of Ag and ZnO nanoparticles using Green synthesis method. Noni has unique medicinal properties which help in normalization of abnormally functioning cells by delivering the bio-chemical nutrients. Synthesized Ag and ZnO nanoparticles have been applied on to jean fabric by dip and cure method. Nanoparticles were characterized by different methods such as UV-VISIBLE, FTIR, SEM, EDS, TEM and a comparative study of antimicrobial activity were done. Application of Ag and ZnO nanoparticles to jean fabric may reduce bacterial infections and blocks UV rays. Zone of inhibition of silver was almost similar to zinc oxide nanoparticles but comparative to silver, zinc oxide was cost effective and less toxic.
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