Objective: PMMA bone cement develop local thrombi, to solve that,our study found that ES-PMMA bone cement,a new kind of material,could reduce local thrombosis.We took a simple and reproducible animal model to confirm the reducing and preliminarily explored the molecular mechanism. Methods: On the basis of carotid artery vein extracorporeal shunt, the New Zealand rabbits were modeled into three groups: Sham group, PMMA group and ES-PMMS group. There were 10 rabbits in each group. Four hours after modeling,collecting the experimental samples,and comparing the amount of thrombosis between those groups. The collected vascular tissue samples were quantitatively detected for endothelial cell related thrombomodulin.Results: There were thrombosis in PMMA group and ES-PMMA grounp,which didn't exist in Sham group. The weight of thrombosis in PMMA group was 0.00732±0.00089g/cm, The weight of thrombosis in the ES-PMMA group was 0.00554±0.00077g/cm,P < 0.001. We found that the expression of CD40, a related protein that can regulate thrombosis in vascular endothelial cells, was significantly lower in the ES-PMMA group than that in the PMMA group using RT-qPCR as well as Western blot.Conclusion: Compared with PMMA bone cement, ES-PMMA bone cement can reduce local thrombosis by decreasing the expression of thrombus associated regulatory protein CD40 in vascular endothelial cells.
Objective: To demonstrate, in a simple and reproducible animal model, that ordinary high viscosity bone cements develop local thrombi; To reduce the occurrence of thrombus in joint arthroplasty, we develop a new material, enoxaparin sodium high viscosity bone cement, to reduce local thrombosis and preliminarily explored the molecular mechanism by which it reduced local thrombus occurrence compared with ordinary high viscosity bone cement. Methods: We used New Zealand rabbits to establish two groups of animal models: ordinary high viscosity bone cement group and new enoxaparin sodium high viscosity bone cement group on the basis of carotid artery vein extracorporeal shunt. There were 3 rabbits in each group. The experimental samples were collected 4 hours after modeling, and the amount of thrombosis between the two groups was compared, The collected vascular tissue samples were quantitatively detected for endothelial cell related thrombomodulin.Results: we successfully established the animal model on the basis of arteriovenous shunt in New Zealand rabbits. We found that both ordinary high viscosity bone cement and new enoxaparin sodium high viscosity bone cement could form thrombosis. The weight of thrombosis in ordinary high viscosity bone cement group was 0.00706 ± 0.00136g/cm, The weight of thrombosis in the new enoxaparin sodium high viscosity bone cement group was 0.00551 ± 0.00115g/cm. The amount of thrombosis in the new enoxaparin sodium high viscosity bone cement group was significantly reduced. We detected by RT qPCR and Western blot that the expression of CD40, a related protein that can regulate thrombosis in vascular endothelial cells, was significantly lower in the new enoxaparin sodium high viscosity bone cement group than that in the normal high viscosity bone cement group.Conclusion: We confirm that the new enoxaparin sodium high viscosity bone cement forms less thrombus than common high viscosity bone cement and preliminarily elucidate that the molecular mechanism by which this new material reduces thrombus formation is to decrease the expression of thrombus associated regulatory protein CD40 in vascular endothelial cells.
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