Objectives:
To investigate the relationship between a prostasin gene variations and the development of preeclampsia in a Pakistani female population.
Methods:
This was a case-control study carried out at University of Karachi, Karachi, Pakistan between May 2018 and 2019. A single nucleotide polymorphism (SNP) at rs12597511 locus was examined with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses in 76 preeclamptic and 74 normotensive expecting mothers.
Results:
We observed significantly increased risk of preeclampsia associated with the CC genotype of rs12597511 polymorphism as compared to TT (
p
<0.001, OR=8.08, 95% CI:1.28-31.19) and TT/TC (
p
<0.001, OR=14.66 and 95% CI: 3.31-65.07) genotypes carriers. Calculation of the allelic distribution revealed a higher frequency of the T allele (82%) among controls; however, the C allele was more prevalent in the preeclamptic group (36%) significantly.
Conclusion:
The significantly higher C allele frequency in the prostasin gene at the rs12597511 locus in the preeclamptic group indicates that the distribution of the C allele of the prostasin gene is a potential risk factor contributing to the development of preeclampsia.
Objective: The study was performed to investigate the association of hypertension in pregnancy with prostasin gene polymorphism in Pakistani females.
Methods: This case-control study was performed at University of Karachi, Pakistan from April 2018 to May 2019. A total of 160 females, including 90 hypertensives and 70 healthy pregnant females, were recruited by purposive sampling after obtaining informed written consent. Genotyping was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
Results: The frequencies of the TC and CC genotypes were higher in hypertensive pregnant females compared to healthy controls. A significant difference was evident for CC (P=0.012) genotype; however, no significant difference was observed for TC (P=0.49) and TT genotypes (P=0.06) between control and hypertensive groups. The adjusted odds ratio for CC genotype was 6.2 (P=0.025) and 1.48 (P=0.44) for TC genotype compared to the TT genotype. There was a significantly higher prevalence of the C allele of the prostasin gene at rs12597511 in the hypertensive group, suggesting that this allele is a risk factor for hypertension and cardiovascular diseases.
Conclusion: C allele at rs12597511 of prostasin gene demonstrate as a risk factor for having hypertension in pregnancy.
doi: https://doi.org/10.12669/pjms.37.1.3666
How to cite this:Ejaz S, Ali A, Riffat S, Azim K, Mahmood A. Genetic polymorphism of the prostasin gene in hypertensive pregnant Pakistani females. Pak J Med Sci. 2021;37(1):109-113. doi: https://doi.org/10.12669/pjms.37.1.3666
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Some of non-pathogenic bacteria are effective biocontrol agents and plant
growth inducers besides its degradative property on polycyclic aromatic
hydrocarbons (PAH). Herein, we report a novel candidate Serratia species
isolated in the purpose of PAH degradation, with its plant-growth-promoting
and antifungal effect against Phytophthora infestans. Properties of
bacterium determined by antifungal and phytostimulation assay under in vitro
conditions displayed production of indole-3-acetic acid (IAA), chitinase and
endoglucanase/cellulase activity. The identification of bacterium using
whole-genome shotgun sequencing output also showed that the novel strain
belongs to new Serratia species harboring the genes responsible for
different secondary metabolites at the genomic level. Genome-wide analysis
suggested a new candidate Serratia species (strain AGBY19) showing, in some
extend, genetic relation with Serratia fonticola at molecular phylogeny
level, which inhibits the growth of phytopathogenic fungi Phytophthora
infestans by 73% compared to the control observed in vitro conditions. This
strain colonised at the rhizosphere of tomato plant during in vivo host
plant cultivation assay that remarkably enhanced the root growth. It causes
the production of IAA hormone and cell wall degrading enzymes (chitinase,
endoglucanase/cellulase). Further genome analyses of AGBY19 revealed
different gene clusters comprising flanked regions associated with the
production of secondary metabolites. These data eventually have provided its
biocontrol properties and plant-growth inducer effect with globally
potential to use for agricultural production.
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