Use of silver nanoparticles (SNPs) for control of implant-associated infection is a promising strategy, if optimum antimicrobial yet nontoxic dose to mammalian cells is identified. This study was done to determine essential quantity of SNPs, which stimulate antimicrobial activity without cytotoxicity, when immobilized on poly (ɛ-caprolactone) (PCL) scaffold proposed for vascular tissue engineering. During SNP synthesis and scaffold preparation, nanoparticle aggregation was protected using poly (ethylene glycol). Transmission electron microscopy was used to characterize SNP size and to detect its mobilization from scaffold to culture medium. Antimicrobial property of the SNP and its dose response was tested using both Gram-positive and Gram-negative bacteria by zone of inhibition assay. Endothelial cells (ECs), the main cell type required for vascular tissue engineering, were grown on scaffolds to identify the nontoxic dose. After seeding EC on scaffolds, cell attachment, spreading, and viability/survival were detected using specific markers by flow cytometric/fluorescence microscopic analysis. Real-time polymerase chain reaction detected effect of SNPs on mRNA expression of selected EC-specific functional proteins. Results suggest that even devoid of antibiotics in the medium, 0.1% (w/w) SNP on PCL scaffold is antimicrobial while nontoxic to EC at cellular and molecular level once cultured on the SNP-PCL scaffold.
Poor cell adhesion, cytotoxicity of degradation products and lack of biological signals for cell growth, survival, and tissue generation are the limitations in the use of a biodegradable polymer scaffold for vascular tissue engineering. We have fabricated a hybrid scaffold by integrating physicochemical characteristics of poly(epsilon-caprolactone) (PCL) and biomimetic property of a composite of fibrin, fibronectin, gelatin, growth factors, and proteoglycans to improve EC growth on the scaffold. Solvent cast porous films of poly(epsilon-caprolactone) was prepared using PEG as a porogen. Porosity varied between 5 and 200 microm, and FTIR spectroscopy confirmed structural aspects of PCL. Films kept in PBS for 60 days showed tensile strength and elongation matching native blood vessel. Slow degradation of the scaffold was demonstrated by gravimetric analysis and molecular weight determination. Human umbilical vein endothelial cell (HUVEC) adhesion and proliferation on bare films were minimal. FTIR spectroscopy and environmental scanning electron microscopy (ESEM) of PCL-fibrin hybrid scaffold confirmed the presence of fibrin composite on PCL film. HUVEC was subsequently cultured on hybrid scaffold, and continuous EC lining was observed in 15 and 30 days of culture using ESEM. Results suggest that the new hybrid scaffold can be a suitable candidate for cardiovascular tissue engineering.
A major constraint in the use of biodegradable polymer scaffolds for vascular tissue engineering is poor cell adhesion and lack of signals for new tissue generation. The presence of extracellular matrix (ECM) within the scaffold is desirable for growth of endothelial cells and in vitro formation of remodelled vascular conduit. In this study, we have produced a hybrid scaffold by coating porous poly-caprolactone (PCL) film with biomimetic ECM components consisting of fibrin, gelatin, fibronectin, angiogenic growth factors and proteoglycans. Human umbilical vein endothelial cells (HUVECs) adhered, spread, proliferated and survived for long periods in culture on the hybrid scaffold. As compared to bare PCL, enhanced cell adhesion, spreading and cytoskeletal organization were demonstrated on the hybrid scaffold, using confocal microscopy of EC-actin stained with Texas red-conjugated phalloidin. Population doubling of endothelial cells (ECs) on the hybrid scaffold and bare scaffold was estimated as 42 h and 136 h, respectively, as assessed by a 3H-thymidine uptake method. Analysis of proliferating cell nuclear antigen (PCNA) also indicated low proliferation on bare scaffold. Flow cytometric analysis of annexin V-stained cells showed poor survival of ECs on bare PCL as compared to the hybrid scaffold. Deposition of insoluble collagen and elastin was identified on the hybrid scaffold by cells recovered after 15 days and 30 days of EC culture, using fluorochrome-tagged specific antibodies and confocal microscopy, and the fluorescence intensity corresponding to elastin and collagen after 30 days was similar to that of 15 days. The results indicate that ECM deposition by endothelial cells is a regulated process without excessive accumulation after 30 days.
Porous and pliable conduits made of biodegradable polymeric scaffolds offer great potential for the development of blood vessel substitutes but they generally lack signals for cell proliferation, survival and maintenance of a normal phenotype. In this study we have prepared and evaluated porous poly(ε-caprolactone) (PCL) integrated with fibrin composite (FC) to get a biomimetic hybrid scaffold (FC PCL) with the biological properties of fibrin, fibronectin (FN), gelatin, growth factors and glycosaminoglycans. Reduced platelet adhesion on a human umbilical vein endothelial cell-seeded hybrid scaffold as compared to bare PCL or FC PCL was observed, which suggests the non-thrombogenic nature of the tissue-engineered scaffold. Analysis of real-time polymerase chain reaction (RT-PCR) after 5 days of endothelial cell (EC) culture on a hybrid scaffold indicated that the prothrombotic von Willebrand factor and plasminogen activator inhibitor (PAI) were quiescent and stable. Meanwhile, dynamic expressions of tissue plasminogen activator (tPA) and endothelial nitric oxide synthase indicated the desired cell phenotype on the scaffold. On the hybrid scaffold, shear stress could induce enhanced nitric oxide release, which implicates vaso-responsiveness of EC grown on the tissue-engineered construct. Significant upregulation of mRNA for extracellular matrix (ECM) proteins, collagen IV and elastin, in EC was detected by RT-PCR after growing them on the hybrid scaffold and FC-coated tissue culture polystyrene (FC TCPS) but not on FN-coated TCPS. The results indicate that the FC PCL hybrid scaffold can accomplish a remodeled ECM and non-thrombogenic EC phenotype, and can be further investigated as a scaffold for cardiovascular tissue engineering.
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