A filamentous bacteriophage (f), fRS603, which is infectious to the phytopathogen Ralstonia solanacearum was isolated. fRS603 was found to have a circular single-stranded DNA genome composed of 7679 nucleotides and to contain 13 putative open reading frames (ORFs). The fRS603 genome showed strong similarity with those of Ralstonia phages fRSM1 and fRSM3, as reported by Askora et al. The fRS603 genome had no ORFs corresponding to ORFs 2, 3, 13 and 14 (integrase) of fRSM3. fRS603 had an ORF that was homologous to other Ralstonia phages fRSS0 and fRSS1; however, fRSM1 and fRSM3 did not.
ϕRS138, a bacteriophage of the family Siphoviridae that lyses Ralstonia solanacearum, was isolated. The genomic DNA of ϕRS138 was 41,941 bp long with a GC content of 65.1 % and contained 56 putative open reading frames. The ϕRS138 genome could be divided into three regions based on similarities to other genomes: (1) a region containing genes encoding a putative transcriptional regulator and an integrase, similar to the prophage genes in Ralstonia solanacearum K60-1; (2) a region encoding proteins related to structural modules and virion morphogenesis, similar to genes in the Pseudomonas phages of the family Siphoviridae; and (3) a region highly similar to the genomes of other Ralstonia solanacearum strains.
Filamentous bacteriophage RS611 (ϕRS611), which infects the phytopathogen Ralstonia solanacearum, had a circular single-stranded DNA genome that was characterized as an Ff-type phage belonging to the family Inoviridae. The ϕRS611 genome was composed of 6386 bases with a G + C content of 62.1 % and contained 11 putative open reading frames. The ϕRS611 genome showed high similarity to those of Ralstonia phages RSS0 and RSS1. However, approximately 900-nucleotide deletions were found in the region corresponding to open reading frames 10 and 11 of ϕRSS0 and ϕRSS1.
The authors would like to publish an erratum to correct the typographical error in the figure legend and text. The sentence from Fig. 1 legend: ''…and that of /RSS1 was constructed with ORF Finder software.'' should read as: ''and that of /RS611 was constructed with ORF Finder software.'' Further, the sentence from second line of right column of the second page: ''ORF 4 (66 aa) of /RS611 lacked 23 amino acids in the N-terminal region compared with ORF 2 (89 aa) of /RSS0 and /RSS1''. Should be: ''ORF 4 (66 aa) of /RS611 lacked 23 amino acids in the N-terminal region compared with ORF 4 (89 aa) of /RSS0 and /RSS1.'' The online version of the original article can be found under
We report magnetization measurement in the superconducting state of a type-II organic layered superconductor with asymmetrical donor k-(MDT-TTF)2AuI2. The demagnetization factor of a plate-like shape of the single crystal has been taken into account for the measurement by applying an external field perpendicular to the conducting plane. The superconducting transition temperature TC is determined to be 4.7 K through the detection of demagnetization signal. The lower critical field is 10.5±1.4 G. This result implies that the stable vortex state of k-(MDT-TTF)2AuI2 can be reached at the applied magnetic field above 105 Oe
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