The Response Surface Analysis using the BBD (Box-Behnken Design) design (Design Expert, Cabit Information Technology Co., Ltd) was applied in identifying the optimal process parameters for the separation and purification of fennel oil using molecular distillation technology. The experiments were carried out based on single factor analysis (feeding rate, evaporation temperature and film wiping speed). The dependent variable second-order regression equation was obtained. The optimal conditions were identified as: feeding rate = 2 drops/second, evaporation temperature = 50°C and film wiping speed = 215 rpm. The concentration of anisole in the extraction under the optimized condition reached 86.69%.
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