This study examined twenty six microsatellite primers developed from three cyprinid fishes (Cyprinus carpio, Barbus barbus and Barbonymus gonionotus) in two indigenous mahseer, Tor douronensis and T. tambroides. A total of 10 (38%) and 12 (46%) primers were successfully amplified producing four and five polymorphic loci in T. douronensis and T. tambroides, respectively. The number of alleles per locus ranging from 2 to 5 and 2 to 7 in T. douronensis and T. tambroides, respectively. A significant deviation from Hardy-Weinberg equilibrium (HWE) was observed at three loci (Barb37, Barb59 and Barb62) in one or more populations in T. tambroides while two loci (Barb37 and Barb62) were deviated in T. douronensis population of Batang Ai. Bayesian cluster analysis performed with STRUCTURE showed that the most likely K value identified was K = 2 with no evidence of population substructuring, similar to those identified by the UPGMA dendrogram. The low genetic distances among populations were also supported by low interpopulation genetic differences (FST) among pairwise populations in both mahseer. Overall, the identified microsatellite loci exhibit promise for use in fine scale population structure analysis of T. douronensis and T. tambroides natural populations.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.