Studies have demonstrated that social media use, as well as problematic smartphone use (PSU), are associated with psychopathology variables, such as depression and anxiety. However, it has not been studied how Instagram use frequency is associated with depression, anxiety, and PSU. The aim of this study was to investigate whether Instagram use frequency is related to these psychopathology variables. Three hundred and five active Instagram users ( Mage = 23.61, SDage = 5.33; 82.2% female) comprised the effective sample in this study. They responded to an online survey that included questionnaires regarding their Instagram and smartphone use, as well as about experiencing depression and anxiety symptoms. We also retrieved objectively measured Instagram use data. The results showed that although Instagram use frequency, depression, and anxiety were associated with PSU in bivariate analysis, Instagram use frequency did not have indirect effects in the relations between psychopathology variables and PSU. Furthermore, while younger age and female sex predicted Instagram use frequency, these socio-demographic variables did not predict PSU. According to our findings Instagram use frequency contributes to PSU, but it is not related to depression and anxiety.