Morphology and life history of 3 cephaline gregarines found in the gut of the pest of stored fruit, Oryzaephilus mercator. are described. Of these 3, 2 are new species. The 3 species are (1) Hirmocystis minuta (Ishii, 1914) (LP TL = 1:7 – 1:22, WP/WD = 1:1, 6–1:7); (2) Amsotobus indicus n. sp. (LP/TL = 1:3–1:6, WP/WD = 1:1 – 1:1.3); (3) Leidyana oryzaephili n. sp. (LP/TL = 1:2 – 1:12; WP/WD = 1:1–1:1.6).
Nonylphenol ethoxylate (NPE), a member of alkylphenol ethoxylates (APEs) is a toxic xenobiotic compound and non-ionic surfactants. It is used widely as a raw material for processing and cleaning in so many factories, mills and industries. In the present study, median lethal concentration (LC 50 ) of NPE was monitored in the Indian minor carp Labeo bata through probit analysis method. Behavioural responses were also observed in this fish upto 96 hours experimental period with the exposure of NPE. The LC 50 value of NPE was followed as 4.90 mg L -1 , 4.79 mg L -1 , 4.68 mg L -1 and 4.47 mg L -1 at 24h, 48h, 72h and 96h respectively for the fish L. bata. In the exposed fishes, excessive mucous secretion was observed over the body surface and also various type of behavioural changes were followed as suffocation, erratic swimming opening of mouth and gill, lethargic movement upward and downward movement and gulping before death. NPE is moderately toxic to the fish L.bata according to its LC 50 values. Behavioural modifications were considered as a stress marker for the Indian minor carp L.bata with the exposure of NPE.
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