Chronic lithium treatment of B-lymphoblast cell lines (BLCLs) from bipolar-I disorder (BD-I) patients and healthy subjects ex vivo attenuates agonist-and thapsigargin-stimulated intracellular calcium (Ca 2 þ ) responses. As these findings suggest that chronic lithium treatment modifies receptor (ROCE) and/or store-operated Ca 2 þ entry (SOCE) mechanisms, we determined whether chronic lithium treatment of BLCLs modified the expression of two members of the transient receptor potential channels (TRPC1 & 3), which participate in ROCE/SOCE. Chronic lithium treatment significantly reduced BLCL TRPC3 immunoreactivity (repeated-measures ANOVA, P ¼ 0.00005), with interaction effects of diagnosis (P ¼ 0.037) and sex (P ¼ 0.040). The lithium-induced decrease was greatest in BLCLs from female BD-I patients compared with those from healthy females (À27%) and with vehicle-treated BLCLs from female BD-I patients (À33%). However, lithium treatment did not affect TRPC1 and 3 mRNA levels, and TRPC1 immunoreactivity. Downregulation of TRPC3 may be an important mechanism by which lithium ameliorates pathophysiological Ca 2 þ disturbances as observed in BD.
Capitalizing on large-scale origin-destination travel surveys conducted in two large Canadian urban centers, Montreal and Toronto, this paper presents a comparative analysis of the travel behavior trends in relation to variables such as demography, car accessibility, home location, and employment status. With trip rate as the dependent variable, three disaggregate multivariate regression models were estimated to observe how behaviors have evolved over time and how individual features affect the way in which people travel. These multivariate models allow the observation of the similarities and differences between explanatory factors between regions and over time. Although both cities are facing similar trends, such as the aging of the population, increasing rates of motorization, declining household sizes, and urban sprawl, they are also the sites of different trends with respect to average trip rates, differences between genders, and the impacts of car access. The geographic and cultural differences found between the populations of Toronto and Montreal include a smaller gender impact on trip generation in Montreal and a smaller age impact in Toronto. The study identified changes in the magnitude of the influence of explanatory variables on trip generation over time, including the declining importance of age and gender. The most promising policy actions that could be taken to decrease trip rates would be those that affect a decrease in household automobile ownership, as determined on the basis of the analysis in this paper.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.