A lipase which hydrolyzes triglycerides (tricaprylin and trilaurin) and naphthyl laurate was obtained from the broth of Corynebacterium acnes cultures by ammonium sulfate fractionation. Ca2+ and sodium taurocholate stimulated activity of the enzyme. Ethylenediaminetetraacetic acid (EDTA) did not inhibit activity of the Ca2+-activated enzyme, but lipolytic activity was inhibited by EDTA in the absence of Ca2+. Tetracycline (10-4M) produced a slight inhibition of the lipase activity with 5 X 10-5 M or less showing no effect on the lipase activity. However, complete inhibition by tetracycline at 104 M was observed for Ca2+-activated enzyme. Tetracycline inhibition of the C. acnes lipase could be demonstrated at concentrations as low as 10-6 M.
A lipase which hydrolyzes triglycerides (tricaprylin and trilaurin) and naphthyl laurate was obtained from the broth of Corynebacterium acnes cultures by ammonium sulfate fractionation. Ca 2+ and sodium taurocholate stimulated activity of the enzyme. Ethylenediaminetetraacetic acid (EDTA) did not inhibit activity of the Ca 2+ -activated enzyme, but lipolytic activity was inhibited by EDTA in the absence of Ca 2+ . Tetracycline (10 −4 m ) produced a slight inhibition of the lipase activity with 5 × 10 −5 m or less showing no effect on the lipase activity. However, complete inhibition by tetracycline at 10 −4 m was observed for Ca 2+ -activated enzyme. Tetracycline inhibition of the C. acnes lipase could be demonstrated at concentrations as low as 10 −6 m .
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.