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Abstract. Large areas of grassland in the agro-pastoral region of northern China were converted into cropland for grain production, and the remaining grasslands are being overgrazed and seriously degraded. The objective of this study was to evaluate how reductions in grazing intensity affect the soil and vegetation characteristics in grasslands of northern China. Soil heterogeneity and vegetation characteristics were evaluated for ungrazed (UG), moderate grazing (MG), and heavy grazing (HG) sites. Grazing increased diversity, but heavy grazing decreased aboveground biomass and increased the non-grass component. The non-grass proportion of total biomass increased with grazing intensity, which was 8, 16 and 48 % for UG, MG and HG sites, respectively. Species richness at the MG and HG sites was significantly higher than at the UG site (P < 0.05) with 3.6, 5.5 and 5.7 for UG, MG and HG sites, respectively. Strong spatial dependence of the examined soil properties at 10 m scale for all grazed sites was revealed by the ratio of nugget to total variation (0-23 %). Overgrazing homogenized soil characteristics at a 10 m scale. The ranges of spatial autocorrelation for soil organic C (SOC) and total N were both > 120 m at the HG site, which was considerably larger than that at the MG and UG sites with corresponding distances of 17.3 and 20.8 m for the MG site and 8.6 and 15.0 m for the UG site, respectively. The sampling density and sampling space for the HG site could be decreased under this scale sampling interval (10 m). Therefore, MG was recommended as the preferred management alternative for grasslands in northern China because of increased plant diversity without negative consequences related to decreased forage quality, forage quantity and soil heterogeneity for the investigated soil properties in northern China's grasslands.

Infectious bronchitis virus (IBV), the causative agent of infectious bronchitis, results in respiratory disease, nephritis, and poor egg production and quality in chicken. Antimicrobial peptides possess potent antimicrobial activities and are regarded as promising therapeutic alternatives in the fight against microorganisms. To assess the in vitro antiviral activity of swine intestine antimicrobial peptides (SIAMP) against IBV, 45 chick embryos were randomly assigned into 3 groups, 15 for each group. Embryos in group 1 were inoculated with IBV. Group 2 was inoculated with SIAMP-IBV intermixture. Group 3 was used as a control and inoculated with sterile PBS. Allantoic fluid was collected for hemagglutination titer assay. In addition, weight gain, mortality, and pathological changes for each group were recorded. The results showed that no distinct pathological changes were found in chick embryos after they were inoculated with SIAMP-IBV intermixture and the mortality was reduced remarkably compared with the IBV-infected group. Weight gain of embryos in the SIAMP-IBV intermixture group was significantly higher than the IBV-infected embryos (P < 0.01), which was also higher than the control group (P < 0.05). Furthermore, the hemagglutination titer in the SIAMP-IBV group was significantly lower than that in the IBV-infected group (P < 0.01). These results indicated that SIAMP can inhibit virus replication and reduce tissue injury caused by IBV.

ABSTRACT:The shape of a live pig is an important indicator of its health and value, whether for breeding or for carcass quality. This paper implements a prototype system for live single pig body surface 3d scanning based on two consumer depth cameras, utilizing the 3d point clouds data. These cameras are calibrated in advance to have a common coordinate system. The live 3D point clouds stream of moving single pig is obtained by two Xtion Pro Live sensors from different viewpoints simultaneously. A novel detection method is proposed and applied to automatically detect the frames containing pigs with the correct posture from the point clouds stream, according to the geometric characteristics of pig's shape. The proposed method is incorporated in a hybrid scheme, that serves as the preprocessing step in a body measurements framework for pigs. Experimental results show the portability of our scanning system and effectiveness of our detection method. Furthermore, an updated this point cloud preprocessing software for livestock body measurements can be downloaded freely from https://github.com/LiveStockShapeAnalysis to livestock industry, research community and can be used for monitoring livestock growth status.

Genetic diversity was investigated for forty populations of ten Elymus species from the Tibetan and Inner Mongolian plateaus of China using allozyme and microsatellite markers. The two assays differed in the amount of polymorphism detected. Micro-satellites detected the higher amounts of polymorphism. For allozymes and micro-satellite markers, between populations of each species, the proportion of polymorphic loci, heterozygosity and Shannon's Information index ranged from 0AE1667 to 0AE833, and 0AE455 to 1AE00, respectively, 0AE0800 to 0AE2528 and 0AE1622 to 0AE3619, respectively, and 0AE1173 to 0AE3769 and 0AE2248 to 0AE5318 respectively. As for gene flow at the species and population levels, limited gene flow was found between populations within species and between species. From a geographical standpoint, samples from different regions showed a range of values. Populations from the Tibetan Plateau of China had higher genetic variation than from the Inner Mongolian plateau both using allozyme and microsatellite markers.

The aim of this study was to investigate mineral metabolism and immune response in dairy goats following intramammary inoculation with varying doses of Staphylococcus aureus. Blood samples were collected at 0, 2, 4, 8, 12, 24, 48, and 72 h after intramammary inoculation. Lowered plasma Fe concentrations were observed from 12 to 24 h postinoculation in groups SAA (Staph. aureus at 10(4) cfu, n = 5) and SAB (Staph. aureus at 10(8) cfu, n = 5). Plasma Cu concentrations increased in group SAB 2 h after inoculation and maintained greater concentrations until the end of the experiment compared with the control group (phosphate-buffered saline, n = 5). Increased plasma Zn concentrations in group SAB were observed 48 h after inoculation, and the concentration was still greater 72 h after inoculation compared with the control group. Greater plasma Mg concentrations were detected in groups SAA and SAB compared with the control group at all timepoints after inoculation. Plasma Mg concentrations were generally greater in group SAA than in group SAB through 72 h (except at 2 h). Plasma tumor necrosis factor-alpha concentrations were unchanged following intramammary inoculation with Staph. aureus throughout the study. Plasma IL-6 concentrations in groups SAA and SAB increased gradually compared with the control group and peaked at 48 h after inoculation. In group SAB, serum cortisol concentrations started to increase from 8 h postinoculation and peaked at 12 h postinoculation. In conclusion, increasing the inoculum dose does not induce more rapid proinflammatory cytokine responses, whereas the data indicate that mineral metabolic alterations occur during the course of Staph. aureus mastitis in the goat.

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