During February 2005, 55 single-spore isolates of Botrytis cinerea were collected at the end of the season from vegetable crops grown in 18 greenhouses on the island of Crete, Greece. They were tested for sensitivity to the anilinopyrimidine fungicides pyrimethanil and cyprodinil, the hydroxyanilide fungicide fenhexamid, the phenylpyrrole fungicide fludioxonil, the dicarboximide fungicide iprodione, and the benzimidazole fungicide carbendazim. Results of the study showed the existence of benzimidazole- and dicarboximide-resistant strains at frequencies of 61.8 and 18%, respectively. Moreover, for first time, the development of resistance to anilinopyrimidine fungicides by B. cinerea was detected in greenhouse vegetable crops on the island of Crete. High resistance frequencies of 49.1 and 57.4% were observed for pyrimethanil and cyprodinil, respectively. In addition, one isolate was found to be resistant to the hydroxyanilide fungicide fenhexamid, while no strains resistant to the phenylpyrrole fungicide were detected. Among the 55 isolates tested, 13 were resistant only to carbendazim, 6 were resistant only to anilinopyrimidines, 3 were resistant to both benzimidazoles and dicarboximides, 17 were resistant to both benzimidazoles and anilinopyrimidines, 6 were resistant to both dicarboximides and anilinopyrimidines, 1 was simultaneously resistant to benzimidazoles, dicarboximides, and anilinopyrimidines, 1 was resistant to both anilinopyrimidines and hydroxyanilides, and 8 were sensitive to all fungicides tested. A strong cross-resistance relationship was found between the two anilinopyrimidine fungicides tested when log transformed EC50 values of the isolates were subjected to a linear regression analysis (r = 0.71). Despite the detection of several phenotypes with simultaneous resistance to chemically unrelated active ingredients, in none of the remaining possible fungicide pairs was there observed any kind of cross-resistance relationship.

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Thirty-five Colletotrichum lindemuthianum isolates were obtained from bean (Phaseolus vulgaris) fields in 23 prefectures of Greece during a survey conducted in 2003 and 2004. Race characterization based on the standardized set of 12 differential cultivars demonstrated the presence of races 2, 6, and 22, which are reported for first time in Greece, while race 22 is reported for the first time in the world. In addition, pathotype diversity showed significant correlation with the geographical origin of these isolates. All three races caused disease symptoms only on cultivars of Andean origin, suggesting that Greek isolates originated from South American countries. Virulence spectrum of the same isolates was also examined on a set of 30 Greek bean cultivars showing seven types of virulence. Based on their reactions to the pathogen isolates, Greek cultivars were divided into nine groups. Among these cultivars, two (Ithomi FS60 and Larisa) were resistant to all tested isolates, including the reference isolates. Molecular diversity was detected using random amplified polymorphic DNA (RAPD) primers and the restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA internal transcribed spacers, which revealed two main clusters of isolates. Thirty-two out of 35 isolates belonged to the same main cluster in both methods, indicating that Greek isolates have genotypic similarities. This study provides the information on population diversity of C. lindemuthianum, which can be useful in agricultural practices and in plant breeding programs.