Background
Enzymatic digestion has been the gold standard for stromal vascular fraction (SVF) isolation but remains expensive while holding practical and legal concerns. Mechanical SVF isolation methods have been known to produce lower cell yields, but may potentially produce a more robust product by preserving the extracellular matrix (ECM) niche.
Objectives
Compare the relative efficacy on wound healing between mechanically-dissociated SVF (M-SVF) and enzymatically-digested SVF (E-SVF)
Methods
Lipoaspirate was partitioned into two equal groups and processed by either mechanical or enzymatic isolation methods. After SVF isolation, cell counts and viabilities were determined by flow cytometry and cell proliferation rates were measured by WST-1 test. Wound healing Scratch Assay test, which is commonly used to model in-vitro wound healing, was performed on both cell cocktails. Collagen type 1 (Col1A) gene expression level, which is known for its role in wound healing, was also measured for both groups.
Results
As predicted, E-SVF isolated more cells (1.74x106/ml [±3.63, n=10, p=0.015]) than M-SVF (0.94x106/ml [±1.69, n=10, p=0.015]), but no significant difference was observed in cell viability. However, M-SVF expressed over 2-fold higher stem cell surface markers and a 10% higher proliferation rate compared to E-SVF. In addition, the migration rate and level of Col1A gene expression of M-SVF were found to be significantly higher than E-SVF.
Conclusion
Although the cell yield of M-SVF was less than the E-SVF, M-SVF appears to have superior wound healing properties.
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