Summary In this paper we investigate the reactivity pattern of T cells from stomach carcinoma patients against autologous tumour cells. T cells obtained from the tumour environment, tumour-draining lymph nodes and peripheral blood were cloned in 78 patients with stomach cancer and anti-tumour cytotoxic T lymphocytes (CTLs) precursor frequencies were assessed in each sample by using limiting dilution analysis. When tumour-specific CTLs were tested for specific T-cell killing by using only low doses of Interleukin 2 (100 U ml-'), a moderate rate of proliferation frequency of T cells (0.047) and specific cytotoxicity (12%) were observed in lymph node populations. When both IL-2 and autologous tumour cells in mixed lymphocyte tumour cultures (MLTCs) were used for stimulation, a dramatic increase in number (0.1) and in specific lytic activity (46%) could be measured. No effect or specific activity to tumour cells was observed with peripheral blood lymphocytes and tumour-infiltrating lymphocytes.
Individual cells of various tissues of Buffalo, Lewis and Sprague Dawley rats were tested for their reactivity with antiserum against Mycoplasma ( M . ) arthrztidzs membranes using the indirect immunofluorescence test. There were considerable differences in the intensity of the fluorescence of the different cell types. A very strong fluorescence was observed with brain cells and chondrocytes.Kidney and heart muscle cells showed strong, liver cells and lymphocytes moderate, and rnacrophages weak fluorescence. There was no difference in the reactivity of the cells of the three rat strains with antiserum against M . arthritidis membranes.
The advantages of electrofusion were used to immortalize the small number of B-cells from fresh biopsy material taken from a gastric carcinoma of a patient. Two stable human antibody secreting clones could be produced which exhibited functional activity against the autologous tumour cells (inhibition of cell adhesion and immunofluorescence staining of the membranes). This shows that a variety of hitherto inaccessible B lymphocyte populations from other human organ biopsies can be immortalised by the improved electrofusion technique.
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