Present investigation deals with the study, to quantify the antibacterial property of silver nanoparticles (SNPs), synthesized by pulsed laser ablation (PLA) in aqueous media, on some human enteropathogenic gram-positive and gram-negative bacterial strains. Antibacterial property was studied by measuring the zone of inhibition using agar cup double-diffusion method, minimum inhibitory concentration by serial dilution method, and growth curve for 24 h. The results clearly show the potency of antibacterial property of PLA-synthesized SNPs and suggest that it can be used as an effective growth inhibitor against various pathogenic bacterial strains in various medical devices and antibacterial control systems.

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A noval derivatives of 3-substituted[1,2,4]triazolo[4,3-b]pyridazine (4a−4o) and 7,8,9,10-tetrahydrobenzo[4,5] thieno[2,3-d][1,2,4]triazolo[4,3-b]pyridazine (7a−7l) is prepared by the reaction of heteroaryl hydrazone from the aldehyde and pyridazinohydrazine derivative, followed by subjecting the intermediate directly to oxidative cyclization employing the mixture of Me4NBr and Oxone. These derivatives were subjected to preliminary antimicrobial activities against microorganism. All these compounds exhibit good to moderate activity.

The present study aimed to investigate the efficacy of flavonoids isolated from the fruit of Terminalia chebula against the hydrogen peroxide-induced DNA damage. Five fractions were screened and were compared for their in vitro antioxidant activity by DPPH method and DNA protection activity by DNA nicking assay. All the extracts showed excellent antioxidant activity with IC 50 values ranging from 11.23 ± 0.18 to 36.70 ± 0.12 µg/ml. The band intensity of supercoiled, circular and linear DNA was measured in DNA nicking assay and it is very clear that the addition of extract significantly inhibited the formation of circular and linear forms of DNA. Methanol-Water 1:1 fraction which showed the highest activity in antioxidant and DNA protection was subjected to structural characterization using different spectroscopic techniques. The structure of the compound was identified as a derivative of luteolin. The result presented in this work strongly supports the hypothesis that DNA protection activities were positively associated with the antioxidant property.

Background: DNA in a human cell is subjected to constant assault from both environmental factors and normal metabolic processes. Accumulation of DNA damage drives the progression of many health disorders like aging, cancer, diabetes, and neurodegenerative disorders. Results: The present study focuses on the isolation of phenolic compound from the fruit of Terminalia chebula and its protective role on induced DNA damage. Diethyl ether and ethyl acetate extract of Terminalia chebula fruit were subjected to column chromatographic purification, and the fractions obtained were tested for the presence of phenolics. Fraction-12 isolated from diethyl ether extract was identified as gallic acid, which is used for cytotoxic and DNA damage protection activity assays. To select a non-toxic concentration of isolated compound, cytotoxicity was assessed by MTT assay. Gallic acid showed moderate toxicity at the highest concentration tested (i.e., percentage cell viability at 100 μg/ml is 40.51 ± 1.31). Antigenotoxic effect of gallic acid on HeLa cells was carried by alkaline comet assay. The compound showed significant protective abilities against hydrogen peroxide-induced DNA damage in HeLa cells. Conclusion: These results show the importance of gallic acid isolated from Terminalia chebula fruit, as protector of oxidative stress-induced DNA damage.

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