The organophosphorus pesticide Phenthoate is one of the most widely used insecticide in agriculture sector to control the varieties of pests and ectoparasites. The acute toxicity bioassay tests were conducted at various concentrations ranging from 0.4 and 4.0 mg/l with an interval of 0.4 mg/l on Indian major carp Labeo rohita for 24, 48, 72 and 96 h. The acute toxicity values were determined as 3.0, 2.6, 2.3 and 2.1 mg/l under laboratory conditions respectively. These LC 50 values showed that the chemical is highly toxic to the fish. Then the fish were exposed to sub lethal concentrations (1/10 th of 96 h LC 50) for 1, 4 and 8 days and studied the significant changes in total proteins and glycogen levels of the fresh wet tissues. The total protein content reduced in various tissues like Liver, muscle, kidney, brain, gill and gut by the percentage of 22.
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