BackgroundPlant Growth Promoting Rhizobacteria (PGPR), Pseudomonas fluorescens strain KH-1 was found to exhibit plant growth promotional activity in rice under both in-vitro and in-vivo conditions. But the mechanism underlying such promotional activity of P. fluorescens is not yet understood clearly. In this study, efforts were made to elucidate the molecular responses of rice plants to P. fluorescens treatment through protein profiling. Two-dimensional polyacrylamide gel electrophoresis strategy was adopted to identify the PGPR responsive proteins and the differentially expressed proteins were analyzed by mass spectrometry.ResultsPriming of P. fluorescens, 23 different proteins found to be differentially expressed in rice leaf sheaths and MS analysis revealed the differential expression of some important proteins namely putative p23 co-chaperone, Thioredoxin h- rice, Ribulose-bisphosphate carboxylase large chain precursor, Nucleotide diPhosphate kinase, Proteosome sub unit protein and putative glutathione S-transferase protein.ConclusionFunctional analyses of the differential proteins were reported to be directly or indirectly involved in growth promotion in plants. Thus, this study confirms the primary role of PGPR strain KH-1 in rice plant growth promotion.
The combination of P. fluorescens strains and B. bassiana isolate effectively reduced the incidence of leaffolder insect and sheath blight disease on rice plants and showed the possibility of controlling both pest and disease using a single bioformulation.
Rice leaffolder Cnaphalocrocis medinalis is a devastating insect pest posing major threat to rice cultivation. Plant growth‐promoting rhizobacteria Pseudomonas fluorescens strain TDK1 was found to induce high degree of resistance in rice plants against leaffolder insect through Induced Systemic Resistance in our previous studies. Thus the present study was aimed to understand the molecular mechanisms involved in P. fluorescens TDK1‐mediated host plant resistance against leaffolder infestation on rice. Transcript profiling was done through DD‐RT‐PCR method in leaffolder‐infested rice plants that were treated with and without Pf TDK1. A total of 3500 transcripts were displayed, of which 165 transcripts exhibited differential expression during rice–Pf TDK1–leaffolder interactions. The differential transcripts were 58 in Pf TDK1‐treated + leaffolder‐challenged, 51 in leaffolderinfested and 56 in Pf TDK1‐treated rice leaf sheath tissues. Annotation and mapping of differentially expressed genes onto metabolic pathways revealed the interaction of leaffolder and Pf TDK1 in rice plant at the molecular level. Furthermore, Pf TDK1 induced over‐expression of genes found to regulate the diverse metabolic processes by the selective integration of defensive signals in rice plants.
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