This study aims to identify an effective carbon source which enhances not only the microbial load but also the shrimps growth. The carbon source cane sugar, molasses and jaggery were tested in 100 L culture tanks with stocking density of 30 mG 2 Peneus monodon seeds for a culture period of 40 days. Jaggery biofloc tank shows highest floc formation (22 mL), highest survival (86%) and highest ABW (6 g), proximate composition of jagerry biofloc had higher protein level (35%) followed by mollases and sugar culture tanks. It confirms jaggery is a new efficient carbon source for shrimp culture system.