A suspension of olfactory epithelial cells was prepared from porcine olfactory mucosa and the physiological functions of the suspension were examined . (a) The membrane potential of the cell suspension, which was monitored by measuring the fluorescence changes of rhodamine 6G, was depolarized by an increase in the K + concentration in the external medium . (b) Various odorants depolarized the cell suspension in a dose-dependent fashion .(c) The magnitude of depolarization by odorants was either unchanged or slightly increased by a reduction of the concentration of Na', Ca 2l , and Cl -in the external medium, which suggests that changes in the permeabilities of specific ions are not involved in depolarization by odorants . (d) The application of various odorants to the cell suspension induced changes in the membrane fluidity at different sites of the membrane that were monitored with various fluorescent dyes [8-anilino-I-naphthalene sulfonate, n-(9-anthroyloxy) stearic acids, 12-(9-anthroyloxy) oleic acid, and (1,6-diphenyl-1,3,5-hexatriene)], which suggests that the odorants having different odors are adsorbed on different sites in the membrane . On the basis of these results, a possible mechanism of odor discrimination is discussed .
Objective: The present investigation aims to develop an efficient, rapid, sensitive, selective, linear, and accurate method for analyzing capecitabine in bulk and tablet dosage form by UV-spectroscopy approaches. Methods: Capecitabine is an estimation by three different developed methods with different UV detection, method A (zero-order spectrophotometric method) at 239 nm, method B (first-order spectrophotometric method) at 231 nm, and method C (area under the curve spectrophotometric method) at 230 to 248 nm. The method's validation and stress degradation studies were done following the International Conference on Harmonization (ICH) guidelines. Results: The methods were validated using the prescribed parameters like system suitability, LOD, LOQ, accuracy, precision, robustness, specificity, etc. The relative standard deviation (% RSD) of the peak area observed in each case was found within the accepted range (<2%). The linearity study's coefficient of correlation (R2) value was<0.99. The methods were quantified accurately in the presence of degraded products. Conclusion: The developed simple and economical method is a suitable option for the qualitative and quantitative study of capecitabine in bulk and tablets, even in its degraded products, which may arise because of oxidation, hydrolysis, thermal, and photolytic decomposition.
A suspension of olfactory epithelial cells was prepared from porcine olfactory mucosa and the physiological functions of the suspension were examined . (a) The membrane potential of the cell suspension, which was monitored by measuring the fluorescence changes of rhodamine 6G, was depolarized by an increase in the K + concentration in the external medium . (b) Various odorants depolarized the cell suspension in a dose-dependent fashion . (c) The magnitude of depolarization by odorants was either unchanged or slightly increased by a reduction of the concentration of Na', Ca 2l , and Cl -in the external medium, which suggests that changes in the permeabilities of specific ions are not involved in depolarization by odorants . (d) The application of various odorants to the cell suspension induced changes in the membrane fluidity at different sites of the membrane that were monitored with various fluorescent dyes [8-anilino-I-naphthalene sulfonate, n-(9-anthroyloxy) stearic acids, 12-(9-anthroyloxy) oleic acid, and (1,6-diphenyl-1,3,5-hexatriene)], which suggests that the odorants having different odors are adsorbed on different sites in the membrane . On the basis of these results, a possible mechanism of odor discrimination is discussed .
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