The cell source for the restoration of injured retina is being intensely studied [1][2][3][4]. In many studies, various models of retina traumas were developed and numerous morphological, immunochemical, and molecular methods were used to determine the changes in precursor cells and their proliferation and differentiation both in vitro and in vivo. These studies demonstrated the existence of stem cells in the fish retina and mammalian ciliary epithelium, multipotent cells in the growth region of amphibians, fishes, and birds, and differentiated cells (pigment epithelium and Müller's glia) that are involved in the regeneration of the amphibian and bird retina.The newt retina is the most popular model for studying retinal regeneration, which is the most pronounced in this species. In the newt, differentiated pigment epithelium cells (PECs) and multipotent cells of the eye growth zone (the pars ciliaris-ora serrata complex) form the source for retina regeneration in situ [5]. In addition, the cells of Müller's glia and microglia, as well as individual cells of the vitreal part of the inner nuclear layer were recently shown to synthesize DNA, whereas replaced bipolars with Landolt club share some morphological and biochemical features with photoreceptors and are assumed to be capable of substituting lost photoreceptors [1].When the cell source for retina regeneration has been previously studied in newts, PEC cultures were grown in the eye cavity ( in oculi ) [6] and in vitro [7]. As a result, fragments of differentiated retina were obtained ( in oculi ), as well as individual types of neuronal populations ( in vitro ). In this study, we used a new method for culturing adult newt retina. The retina cleared from PECs was grown as a long-term in vitro organotypic culture under rotated on a mini-roller. This method is often used in neurotoxicological and physiological studies to maintain the embryonic brain tissue and mammalian retina [8] and to study retina regeneration in reaggregated suspensions of bird retinal cells [9].The amphibian retina has never been cultivated by this method so far. Our approach showed that the retina of an adult newt Pl. waltl remains viable in vitro for a long time. Although some neurons died, retinal cells of the internal regeneration source proliferated, migrated, and populated the nuclear retina layers for four weeks. In addition, these blast cells sometimes aggregate in the cavities of spheroid formed by the retina.Adult Pl. waltl newts were obtained in an aquarium chamber of the Institute of Developmental Biology, Russian Academy of Sciences. The animals were kept and anesthetized, and their eyes were isolated, according to the guidelines of the Russian Academy of Sciences for handling lower vertebrates. Retinas deprived of PECs, choroids, and scleras were isolated from 12 eyes in a culture medium by microsurgery (an incision along the limbus under a binocular magnifier). The samples were placed into vials containing medium 199, bidistilled H 2 O, 1 M HEPES, a solution of gentamycin sulf...