For the e.cient application of haploid induction procedures in cabbage breeding\ a su.cient number of regenerants should be achieved in a broad spectrum of genotypes[ However\ the majority of genotypes are somewhat recalcitrant[ The e.ciency of microspore culture was tested by crossing a responsive "17[6 embryos per Petri dish# and a non! responsive "9[0 embryo# cabbage cultivar[ The embryo yield of one progeny was intermediate "07[8# while two were superior to the best parent cultivar "41[8 and 53[9 embryos#[ Thus\ genes for haploid embryogenesis\ present in responsive lines\ can be e}ectively trans! mitted to responsive × non!responsive hybrids[ Abscisic acid!induced desiccation of embryos was used for the e.cient regeneration of plants[ High germination percentages "43[6Ð69[5)# followed by normal plan! tlet development were achieved[ Spontaneous genome doubling mea! sured at the plantlet stage di}ered markedly in untreated genotypes[ The percentage of diploids ranged from 10 to 56)[ The e}ects of two antimitotic drugs applied to freshly isolated microspores were deter! mined in two experiments[ In the _rst experiment\ tri~uralin "9[4 and 0[9 mg:l# had no e}ect on embryo induction while oryzalin partly "9[014Ð9[14 mg:l# or completely "9[4 mg:l# inhibited the formation of embryos[ In the second experiment\ higher concentrations of tri~uralin increased the proportion of diploidized plants[ Application of anti! mitotic drugs to microspores did generally not improve the overall production of haploid plants\ which was higher in an untreated control[
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