The aim of this study was to investigate the phytochemical constituents, gas chromatography-mass spectrometry (GC-MS) analysis and antimicrobial activity of Coleus forskohlii. The different solvents such as ethanol, chloroform, acetone and aqueous extracts were identified pharmacologically as important bioactive compounds and their antimicrobial properties were studied. In the phytochemical investigation almost all the ethanol extract of leaf, stem and root having secondary metabolites like alkaloids, flavonoids, tannins, saponins, terpenoids, and steroids. The active constituents of the ethanol extract of C. forskohlii root was studied by GC-MS analysis. According to the antimicrobial results ethanol extract of C. froshkolii root showed highest antibacterial activity compared with stem and leaf. The highest antimicrobial activity was observed against Klebsiella pneumonia (19 mm) and Candida albicans (16 mm) in ethanol extract of root. Among the above extracts of leaf, stem and root, ethanol extract of root having antimicrobial activities due to the presence of phytoconstituents.

The rhizosphere of plant possesses important microflora, which secretes wide chemical compounds including secondary metabolites necessary for plant growth and development. The microbial flora of alfalfa plant rhizosphere soil region was explored for functional activity and we found upto ten different pigmented colonies. Due to good functional diversity, this yellow pigmented colony was taken for further studies. Thus, the culture was molecularly characterized and identified for potent bioactive components responsible for antimicrobial activity. The selected culture mass was cultured and secondary metabolites were produced and extracted using ethyl acetate and subjected to GC-MS analysis. The antimicrobial study revealed selective activity against Streptococcus pneumonia, and Proteus sp with zone of inhibition to be 18 and 20 mm respectively.  Molecular identification of the isolate by 16S rRNA sequencing showed the isolate as Macrococcus equipercicus with 100 % similarity. Based on GC-MS analysis report 25bioactive compounds were identified and 13-docosenamide, hexadecanoic acid esters and quercetin were found in ethyl acetate extract. Conclusion: Thus the yellow pigmented gram positive cocci M.equipercicus isolated from Medicago sativa possessed wide antibacterial activity due to presence of quercetin. Through the studies, we were able to identify potent antibacterial compound producing bacteria from M. sativa plant rhizosphere soil.

ABSTRACT:Textiles are used in different sectors and various purposes beyond imagination. Medical sector is one of them. An important and emerging part of the textile industry is medical, hygiene and health sector. The medical textile industries have diversified with new materials and innovative designs. Recently, the application of textiles has on track going beyond the usual wound dressings, incontinence pads and plasters etc. Among these vast categories of medical textile products, the hospital textiles play an important role and also expected to fulfill the hygienic, comfort and microbial resistance property requirements. These functional requirements of hospital textiles have led to the innovative use of a variety of natural fibers with enhanced comfort and hygienic properties in the development of new products for medical applications. The Agave Americana plant is one of the very important natural resource which is available in the tropical areas with plenty of hygienic properties. Based on the view, the Americana plant leaves are collected and extracted by mechanical process. After the extraction the fibers are converted into yarn and fabric forms for the different medical end uses. The extraction was done by using the Box and benkhen optimized process conditions. The anti-bacterial property was tested on fabric using standard AATCC 147 qualitative and AATCC 100 quantitative tests against both positive and negative bacteria with 24 and 48 hour time duration. The anti-bacterial property of Agave Americana fabric was also compared with standard antibiotics. The test result shows that the Agave Americana fiber has good antibacterial activity against the gram negative Escheirchia Coli (32mm & 42mm) bacteria than gram positive Staphylococcus aureus (23mm &35mm) bacteria. Agave Americana fiber has showed higher zone of inhibition against standard antibiotics. Based on the results, the developed anti-bacterial Agave Americana fabric will be most suitable for medical and health care applications.

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