ArticleA f a c i l e g r e e n s y n t h e s i s o f s i l v e r nanoparticles using the medicinal plant Leucas aspera and their antibacterial activity
AbstractThe junction of nanotechnology and biomedical sciences unfasten the possibility for a wide range of biological research topics and medical uses at the molecular and cellular level. In the present study silver nanoparticles were synthesized by using Leucas aspera leaf extract support of biological process. The extract incubated with 1mM AgNO 3 solution showed gradual change in the color of the extract from greenish to reddish brown. Reduction of silver ions by the leaf extract was examined by UV-visible spectroscopic technique. Further, it was confirmed by Fourier Transformations Infrared spectroscopic (FTIR) analysis. The purity of the synthesized silver nanoparticles was examined through X-Ray Diffraction (XRD) analysis. The nanoparticles were examined for the antibacterial activities of antibiotic (Tetracycline), silver nanoparticles and the combined effect of both antibiotic and nanoparticles against the multi drug resistant human pathogens.
Keywords:
Organic synthesis generally required large amount of solvent, avoiding the use of organic solvents in synthesis is a paradigm shift directed at developing more benign chemistry, and with ionic liquids surprisingly can lead to access to new compounds. An elegant one-pot synthesis of quinoline derivatives has been achieved by reaction of substituted anilines with β-ketoester at 60 • C in ethanol using an ionic liquid [Et 3 NH] + [BF 4 ] − as catalyst. All the reactions gave products with high degree of purity and excellent yield (78-93%) within the shorter span of time (20-65 min) than those reactions with conventional methods. The screening of solvents as well as the reuse of ionic liquid has been evaluated. The structure of the products has been elucidated by spectral and analytical data. The present scope and potential economic impact of the reaction are demonstrated by the synthesis of substituted quinolines. Remaining challenges and future perspectives of the new transformation are discussed.
Ionic liquids are exceptional chemical compounds that are used in many areas of
modern science. Due to their highly adjustable nature and incomparable properties, ionic liquids
have become key players in the areas of synthesis, catalysis, extraction, electrochemistry,
analysis, biotechnology, etc. In addition to the physical and chemical properties of ionic liquids,
their high biological activity has attracted considerable attention from biochemists, ecologists
and medical professionals. Due to the properties mentioned above, an imidazolium ionic liquid
solvent was used for the synthesis of nanostructure nanoparticles. In the present work, cubic
form of pure Fe2O3, spherical nature of pure Ag (0) and hexagonal shaped hybrid Ag- Fe2O3
were successfully synthesized by a simple co-precipitation, reduction and co-precipitation
processes. The synthesized nanoparticles were characterized in detail by spectral studies such as
IR, XRD, SEM, EDX and TEM analysis. The size and shape of the particles were performed by
X-ray Diffraction (XRD), Scanning Electron Microscope (SEM) techniques and Transmission
Electron Microscope (TEM) techniques. Quality quantity and purity of the products was checked
by Energy Dispersive X-Ray Analysis (EDX).
Apoptosis, a physiological mechanism of highly orchestrated cell death, can be initiated by extracellular and intracellular mechanisms that trigger a complex machinery of proapoptotic proteases and mitochondrial changes, leading to the activation of specific endonucleases and DNA fragmentation. The present study was undertaken to elucidate a mechanism underlying the inhibitory effect of biosynthesised silver nanoparticle on TNF-α induced NF-κB nuclear translocation in prostate cancer PC- 3 cells. The cell cycle analysis of Prostate cancer PC-3 cells was examined by flow cytometry by using annexin V-FITC/PI staining. Effect of silver nanoparticles in oxidative stress ROS, Effect of biosynthesized silver nanoparticle on apoptosis in human prostate cancer cell line and apoptotic induction of TNF-α and NF-κB expression was studied by Flow cytometry in Prostate cancer PC-3 cell line. From the results it was observed that biosynthesized silver nanoparticle inhibits the cellular growth of human prostate cancer PC-3 cells and induces apoptosis. The ROS levels generated in response to silver nanoparticles were significantly higher in treated PC-3 cells than the control. The result indicates that cell death is mediated by ROS production, which might alter the cellular redox status, and it is a potential reason for cell death. Apoptosis of the silver nanoparticle treated PC-3 cells was accompanied by a reduction in the percentage of cells in G0/G1 phase and an increase in the percentage of G2/M phase cells, indicating cell cycle arrest at G2/M phase, and transcription factor NF-κB plays an essential role in inflammation and cancer. The activation of NF-κB in response to inflammatory cytokine such as TNF-α promotes nuclear migration to enable DNA-binding activity and facilitate target genes expression.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.