The aim of study was to compare yield, fruit quality, chemical composition and fruits colour of berries of two blue honeysuckle (Lonicera caerulea L.) cultivars of Polish bred 'Wojtek' and 'Brązowa' collected at the beginning and at the end of harvest season, respectively. On average, 'Wojtek' yielded 1565 g per bush, whereas 'Brązowa' 1941 g. During the fruit ripening process, considerable changes were found in the fruit colour. Fruits of both cultivars became darker and their colour changed from green and yellow to red and blue. With the changes in fruit colour, considerable changes in firmness also occurred. Fruit ripening reduced fruit firmness and puncture resistance. For both genotypes, the berries of late harvest were bigger and richer in soluble solids and total polyphenol, however, they showed decreased firmness, lower acidity, and L-ascorbic acid content. Nevertheless the time of harvest, 'Wojtek' berries surpassed 'Brązowa' fruit regarding soluble solids, titratable acidity and L-ascorbic acid content (for late-crop berries of both cultivars 14.1>12.6%, 3.4>2.7 g citric acid 100 g 1 , and 76>56 mg 100 mL ). Among phenolics, anthocyanins made the substantial group ranging from 94.47 mg 100 g -1 ('Brązowa' berries of early harvest) to 141.96 mg 100 g -1 (late 'Wojtek' fruit).

Using the RAPD method and bulked segregant analysis we identified four RAPD markers linked to a dominant gene Ns, responsible for a hypersensitive reaction of potato (Solanum tuberosum L.) to potato virus S (PVS) infection. The markers OPEI5550, OPJ 135oo,OPGl7450 and OPH19900 were found to be closely linked to the Ns gene in diploid potato clones. They are situated at 2.6, 3.3, 4.6 and 6.6cM from Ns, respectively. As a source ofthe gene, clone G-LKS 678147/60, which is known to carry Ns transferred from 5. tuberosum ssp. andigena was used. These RAPD markers were not amplified in resistant tetraploid clones containing Ns derived from the clone MPl 65 118/3, also having an andigenum origin. This suggests that there may be two separate loci of A^. s in the sources identified, or different alleles with the same specificity at a single locus, or that the genetic background of tetraploids tested results in different RAPD amphfication patterns.

A high level of PLRV resistance has been found in four diploid genotypes originating from resistant ancestors widely utilized in European potato breeding. Plants of these genotypes were difficult to infect not only with aphids, but also with graft inoculation. Their resistance is associated with limited virus spread, but not with intolerance.

No abstract

No abstract