N5-Substituted formamidopyrimidine
adducts have been observed from the reaction of dGuo or DNA with aziridine
containing electrophiles, including nitrogen mustards. However, the
role of substituted Fapy-dGuo adducts in the biological response to
nitrogen mustards and related species has not been extensively explored.
We have developed chemistry for the site-specific synthesis of oligonucleotides
containing an N5-nitrogen mustard Fapy-dGuo
using the phosphoramidite approach. The lesion was found to be a good
substrate for Escherichia coli endonuclease
IV and formamidopyrimidine glycosylase.
Diamond ultramicroelectrode arrays (D-UMEAs) have been fabricated and evaluated experimentally for chemical sensing. D-UMEAs were found to demonstrate higher current density per unit active area than planar diamond macroelectrode and glassy carbon electrode. D-UMEAs could address new operating envelopes in electrochemical detection, and provide stable sensor electrodes for bio and extreme environments.I.
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