Imaging the microcirculation is becoming increasingly important in assessing life-threatening disease states. To address this issue in a highly light absorbing and light scattering tissue, we use laser scanning multiphoton microscopy and fluorescent 655-nm 5000-MW methoxy-PEGylated quantum dots to image the functional microcirculation deep in mouse hind limb skeletal muscle. Using this approach, we are able to minimize in vivo background tissue autofluorescence and visualize complete 3-D microvascular units, including feeding arterioles, capillary networks, and collecting venules to depths of 150 to 200 microm. In CD1 mice treated with lipopolysaccharide to model an endotoxemic response to bacterial infection, we find that these quantum dots accumulate at microvascular bifurcations and extravasate from the microcirculation in addition to accumulating in organs (liver, spleen, lung, and kidney). The quantum dots are cleared from the circulation with a first-order elimination rate constant seven times greater than under normal conditions, 1.6+/-0.06 compared to 0.23+/-0.05 h(-1), P<0.05, thereby reducing the imaging time window. In vitro experiments using TNFalpha treated isolated leukocytes suggest that circulating monocytes (phagocytes) increased their nonspecific uptake of quantum dots when activated. In combination with multiphoton microscopy, quantum dots provide excellent in vivo imaging contrast of deep microvascular structures.

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This paper examines the levels, trends, patterns and Kuwaitinon-Kuwaiti differentials in fertility, by socioeconomic variables and the major factors that may account for recent changes. Kuwaiti fertility is very high: in 1980, the crude birth rate was about 47 and the total fertility was 6-8 per woman. Childbearing still extends to later ages but fertility in the early and middle reproductive years has declined recently, as a result of the rise in age at marriage and the fall in the proportion of currently married women in the younger age groups. Non-Kuwaiti fertility is consistently lower, with total fertility at about 69% of the Kuwaiti level. This differential cannot be explained by the age structure of the populations alone. Analysis of completed fertility by education and economic activity status indicates an inverse relationship.

SummaryThis paper examines the levels, trends and Kuwaiti–non-Kuwaiti differentials in stillbirth, infant and child mortality rates during the 1957–79 period. The present infant mortality rate (33 per 1000) and its component parts are high in contrast to those in more developed countries. But during the last few decades, the rates showed definite decline. The decline in infant and child mortality was rapid between 1955 and 1970 when the infant death rate was about 100 or more per thousand livebirths, but slowed after the infant mortality rates were brought down to around 50 in 1970. The large scale reduction in mortality since 1950 is closely associated with socioeconomic progress and improvements in standards of living as well as wider availability and better accessibility of health services. Kuwait is still in a position where mortality can be reduced further, provided that investment in health and education continues.

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