Indonesia is a country with vast agricultural land, especially rice fields. Unfortunately, rice crops cause a problem of rice straw waste. The waste of rice straw consists of 38% cellulose, 24% hemicellulose and 8% lignin. The study aimed to assess the production of cellulose by Serratia marcescens in straw substrate medium. The research design was Completely Randomized Design. Variations of rice straw substrate were denoted by (S0; S1; S2; S3) within incubation period for 16 hours. Data analysis was analyzed using SPSS one-way
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NOVA (α = 0.05). The result showed that variations of rice straw substrate concentrations correlated with the production of cellulose at 0.28 U/mL. The result of statistical analysis using DMRT Test showed a significant difference in every treatment (p≤0.056)
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