SummaryIn dengue virus infected Aedes albopictus cells, electron-dense particles, larger than single ribosomes, were arranged on the cytoplasmic sides of rough endoplasmic reticulum (I~ER) membranes. Mature virions 40---45 nm in diameter as well as vesieulotubular structures 50--120 nm in diameter appeared in enlarged cisternae of I~EI~ filled with fine granular substance. Many of the mature virions and somewhat degenerated vesiculotubular structures remained to be enclosed in membranous structures presumably derived from I~.EI~, even after degeneration of infected cells. The findings suggest that development of dengue viruses in cultured A. albopictus cells takes place in close relationship with the activated membranes of RELY.Other morphological changes observed in dengue infected A. albopictus cells were 1. electron-dense "double-track structures" in areas of virion morphogenesis, 2. fine crystalline structures in type-2 dengue infected cells, and 3. aggregates of nucleoid structures, in cells persistently infected with type 2 dengue virus. The implication and nature of these structures in relation to virion morphogenesis remain to be investigated.
Lysis of virus-infected L929 target cells transfected with the H-2 class II IA' gene by class II-restricted influenza virus-specific murine cytotoxic T lymphocyte (CTL) clones was studied by electron microscopy and compared with lysis of L929 cells by class I-restricted CTL clones. T lymphocytes predominantly approached the basal surface of target cells grown on a plastic dish and also approached uninfected L929 target cells, although virus maturation exhibited no polarity with respect to the cell surface site. After incubation for 30 min, the target cell nuclei began to change: chromatin became irregularly redistributed and aggregated, and the nuclei appeared swollen. Later, electron-dense and-light areas of nuclei became segregated, and the cytoplasm became disorganized with many vacuoles. The ultrastructural changes of target cells during lysis by class Iand class 11-restricted CTL clones appeared to be similar. These findings and other cytotoxicity data of class I and class II CTLs are discussed.
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