The fosfomycin resistance enzymes, FosB, from Gram-positive organisms, are M2+ dependent thiol tranferases that catalyze nucleophilic addition of either L-cysteine (L-cys) or bacillithiol (BSH) to the antibiotic, resulting in a modified compound with no bacteriacidal properties. Here we report the structural and functional characterization of FosB from Bacillus cereus (FosBBc). The overall structure of FosBBc, at 1.27 Å resolution, reveals that the enzyme belongs to the vicinal oxygen chelate (VOC) superfamily. Crystal structures of FosBBc co-crystallized with fosfomycin and a variety of divalent metals, including Ni2+, Mn2+, Co2+, and Zn2+, indicate that the antibiotic coordinates to the active site metal center in an orientation similar to that found in the structurally homologous manganese-dependent fosfomycin resistance enzyme, FosA. Surface analysis of the FosBBc structures show a well-defined binding pocket and an access channel to C1 of fosfomycin, the carbon to which nucleophilic addition of the thiol occurs. The pocket and access channel are appropriate in size and shape to accommodate L-cys or BSH. Further investigation of the structures revealed that the fosfomycin molecule, anchored by the metal, is surrounded by a cage of amino acids that hold the antibiotic in an orientation such that C1 is centered at the end of the solvent channel positioning the compound for direct nucleophilic attack by the thiol substrate. In addition, the structures of FosBBc in complex with the L-cysteine-fosfomycin product (1.55 Å resolution) and in complex with the bacillithiol-fosfomycin product (1.77 Å resolution) coordinated to a Mn2+ metal in the active site have been determined. The L-cysteine moiety of either product is located in the solvent channel, where the thiol has added to the backside of fosfomycin C1 located at the end of the channel. Concomitant kinetic analyses of FosBBc indicated that the enzyme has a preference for bacillithiol over L-cysteine when activated by Mn2+ and is inhibited by Zn2+. The fact that Zn2+ is an inhibitor of FosBBc was used to obtain a ternary complex structure of the enzyme with both fosfomycin and L-cysteine bound.
Global networks of the cytosolic glutathione S-transferases illuminate sequence-structure-function relationships across more than 13,000 members of this superfamily, including experimental confirmation of enzymatic activity for 82 members and new crystal structures for 27.
DEAD-box RNA helicases are enzymes that unwind RNA duplexes and are found in virtually all organisms. Most organisms harbor multiple DEAD-box helicases, suggesting that these factors participate in distinct aspects of RNA metabolism. To define the individual and collective contribution of the five DEAD-box helicases in the bacterium Escherichia coli (E. coli ), nonpolar deletion mutants lacking single or multiple DEAD-box genes were constructed. An analysis of the single-deletion strains indicated that the absence of either the DeaD or SrmB RNA helicase causes growth and/or ribosomal defects under typical laboratory growth conditions. The analysis of strains lacking multiple DEAD-box genes showed cumulative growth defects at low temperatures. A strain deleted for all five DEAD-box genes was also constructed for these studies, representing the first time all DEAD-box genes have been removed in any organism. Additional investigations revealed that the growth and ribosomal defects of such a DEAD-box deficient strain can be sharply attenuated under alternative conditions, indicating that the defects caused by a lack of DEAD-box genes are modulated by growth context.
The
Gram-positive pathogen Staphylococcus aureus is a
leading cause of global morbidity and mortality. Like many
multi-drug-resistant organisms, S. aureus contains
antibiotic-modifying enzymes that facilitate resistance to a multitude
of antimicrobial compounds. FosB is a Mn2+-dependent fosfomycin-inactivating
enzyme found in S. aureus that catalyzes nucleophilic
addition of either l-cysteine (l-Cys) or bacillithiol
(BSH) to the antibiotic, resulting in a modified compound with no
bactericidal properties. The three-dimensional X-ray crystal structure
of FosB from S. aureus (FosBSa) has been determined to a resolution of 1.15 Å. Cocrystallization
of FosBSa with either l-Cys or
BSH results in a disulfide bond between the exogenous thiol and the
active site Cys9 of the enzyme. An analysis of the structures suggests
that a highly conserved loop region of the FosB enzymes must change
conformation to bind fosfomycin. While two crystals of FosBSa contain Zn2+ in the active site, kinetic
analyses of FosBSa indicated that the
enzyme is inhibited by Zn2+ for l-Cys transferase
activity and only marginally active for BSH transferase activity.
Fosfomycin-treated disk diffusion assays involving S. aureus Newman and the USA300 JE2 methicillin-resistant S. aureus demonstrate a marked increase in the sensitivity of the organism
to the antibiotic in either the BSH or FosB null strains, indicating
that both are required for survival of the organism in the presence
of the antibiotic. This work identifies FosB as a primary fosfomycin-modifying
pathway of S. aureus and establishes the enzyme as
a potential therapeutic target for increased efficacy of fosfomycin
against the pathogen.
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