When native herbivores are enclosed in fenced reserves without predators or dispersal options then overgrazing can occur, leading to damage to vegetation and co-occurring fauna species. One-way gates that allow medium-sized herbivores to exit fenced reserves may be an effective management tool to address overabundance or facilitate population expansion. We tested the use of one-way gates to facilitate the movement of the reintroduced burrowing bettong (Bettongia lesueur) from inside to outside a fenced reserve in arid South Australia. Oneway gates were installed in the exterior fence of the reserve and assessed using remote motion-sensor cameras. The influence of gate position (dune, swale or corner) and provision of food were assessed in relation to gate visits and exits. Animals were trapped inside and outside the gates to determine any population bias in gate exits. Baited gates recorded significantly more exits than unbaited gates and dune gates had higher exit rates than interdunal swale gates. When gates were unbaited, those installed in corners of the reserve showed significantly higher visitation by bettongs and a non-significant trend towards more exits compared to gates placed in straight sections of fence along dunes or swales. There was no sex or age bias of burrowing bettongs using the gates and bettongs travelled between 75 m and 1535 m from their warrens to use the gates. No non-target species gained access to the reserve through the one-way gates and only two non-target animals used the gates to exit the reserve confirming gate specificity for bettongs. During the same period, 96 burrowing bettongs exited the reserve through the one-way gates. One-way gates may be a management strategy for facilitating passive movement of medium-sized herbivores outside of fenced reserves for the purposes of reducing overpopulation or facilitating population expansion outside reserves.One-way gates were based on a combination of two gate designs tested during pen trials by Crisp and Moseby

No abstract