Microalgae with high oil content are only alternative for decreasing fossil fuel supplies, but more work remains to be done to improve the lipid content of microalgae strains. In this study, strain improvement is done using microwave radiation in Scenedesmus obliquus to increase the production of triacylglycerol, which is the main source of biodiesel. Microalgal culture were exposure to varied microwave irradiation over different time periods. Maximum increase of 2.22-fold in biomass and 2.5-fold in triacylglycerol was observed for microwave irradiation of 25mins and 20mins respectively. The percentage of some monounsaturated fatty acids increased in gas chromatographic examination of neutral lipid fractions from total lipids of microwave irradiated samples, which is considered as one of the preferable properties of biodiesel.
Mammalian system have a large genome with a high level of gene sequence identity from other genomic DNA, making assessment difficult and time-consuming.Our findings describe a simple method for rapidly isolating and amplifying HoxA loci in the mouse genome using degenerate primers. For the semi degenerate primers,they were designed based on cognate gene coding regions of consensus sequences. After assembling sequences from different primer matches amplifying the same HoxA loci, the effects of the universal primer-template match on the efficiency of standard PCR amplification were investigated. Touchdown PCR increased specificity and yield in two consecutive amplifications on different gel concentrations by using high and low annealing temperatures. This method was quick, simple, and inexpensive for amplification of consensus sequences in very large gene sequences.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.