Cassava starch production waste (cassava pulp) has been proposed as a high potential ethanolic fermentation substrate due to its high residual starch level and the small particle size of the lignocellulosic fibers. Saccharification of the residual starch from a 3% (w/v) dry weight basis (DS) of cassava pulp by α-amylase (100°C, 10 min) and glucoamylase (60°C, 2 h) resulted in a glucose yield of 22.6 g/l [67.8% (w/w) DS of cassava pulp] and in lignocellulosic fibers at 0.5 g/g DS cassava pulp. Pretreatment of the lignocellulosic fiber with dilute sulfuric acid and calcium hydroxide at 121°C, 15 lb/in 2 for 30 min increased and decreased, respectively, its susceptibility to cellulase hydrolysis. Under the optimal conditions found, pretreatment of 6% (w/v) DS lignocellulosic fiber by 2% (w/v) H 2 SO 4 for 30 min, followed by saccharification by cellulase (40°C, 9 h), yielded a glucose level of 26.6 g/l [79.8% (w/w) DS of the cassava pulp]. The starch and lignocellulosic fiber hydrolysates obtained from 30 g cassava pulp and 60 g H 2 SO 4 pretreated lignocellulosic fiber were fermented by Saccharomyces cerevisiae, without the need for (NH 4 ) 2 SO 4 supplementation, to yield ethanol levels of 9.9 and 11.9 g/l, respectively, after 48 h.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.