Aims: To verify whether the nanoencapsulation of 15d-PGJ 2 in poly(D,L-lactide-co-glycolide) (PLGA) nanocapsules (15d-PGJ 2 -NC) might potentialize its antinociceptive activity into rats' temporomandibular joint (TMJ). Main methods: Transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to evaluate the morphology and suspension of the PLGA nanocapsules. Rats were pretreated (15 min) with an intra-TMJ injection of unloaded 15d-PGJ 2 or 15d-PGJ 2 -NC at concentrations of 10, 100 or 1000 pg followed by an ipsilateral intra-TMJ injection of 1.5% formalin. The nociceptive behavioral response was observed during 45 min; animals were then sacrificed and the periarticular tissue was removed for IL-1β measurements. Key finding: TEM and AFM analyses showed that 15d-PGJ 2 -NC is spherical without any aggregates or adhesion confirming that this formulation is a good drug carrier system for 15d-PGJ 2 . Pretreatment with 15d-PGJ 2 -NC (100 and 1000 pg/TMJ), but not unloaded 15d-PGJ 2 , was found to significantly decrease the release of IL-1β cytokine and the animals' nociceptive behavioral response induced by intra-TMJ injection of formalin. Significance: The compound 15d-PGJ 2 -NC might be used as a potential antinociceptive and anti-inflammatory agent to treat temporomandibular disorders in clinical practice.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.