Repo rt s of investigations on the effects of applied cAMP (cyclic 3',5'-adcnosinc penetrates the tissues and enters the cells. All conclusions which have been drawn on the effcct or lack of etTect of cAMP were based only on the assumption tha t cAMP penetrated the cell membrane [2 \. Using leaf tissues a nd isolated mesophyll protoplasu of Pelunia hybrido as well as leaf tissues o f ImpaliellS SU/llmi we have now shown that applied cA MP does enter cells, where it is rapidly degraded. Intaet leaftissucs were fed cAMP through their petioles (24 h) or through the leaf surface by vacuum infiltration (30 min).Proloplasts were prepared and used as previously described (4]. Leaf tissue and protoplasts were cx traeted and cAMP conten t detcrmined as repo rtcd earlier (5). Each experiment
SummaryWhen c.urp is red to green J)lant ti ss lles it is capable of entering cell s and is dcgraded 38 rapidly in isolatcd protopla sts ilS in intal't tissucs. Effcets of cAMP on ca rbohydrate storage are fount! when cOllccntra t ions execeding 8· 10-3 ~[are uscd; th c rate of COz-fixation is influenced by > 2· 10-4 )[ crUIP. No effeets on lipid fatty acid l'omposition und mctaboli sm could be cstablish~d, but, after a ppli cat ioll 01 > 4, 10-' ){ f AJIP, thc in corporat ion 01 P'C]+ucetutc into lipids is raised .tpproxi.mately by 88 %' Also, f rUf P lowen; tbc formation of leaf·n ecroses (':\Used by the detergcnt Tween ~O, All effects ea used by l'A~fP ('oulll also be obtained by feeding ö'·,UIP, therefore these effects art' non-spedfic and therc is HO indi ('a tion of a hormon e-mimiced effi cal'Y 01 the cyclic nucJ cotide, In most t ases a d istinct cffed neetl s a cA)l!' concentratioll bigher than 10 4 )[, and with r egarrl to the rapill uptake, it seems probable that it is duc tu an inflllem'c on thc adenylatc pool oi the cell.However, so me rather spcd fif (' :UIP effects descrilJed in th e literature cannot be intHpreted in such a mann er.Einleitung
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