The association constants of the binding of chlorpromazine and imipramine to serum albumin at low saturation of the protein were determined by a new experimental approach with the protein concentration rather than the ligand concentration being varied. This approach is suitable for estimating binding constants in systems with one class of binding sites. In addition, the method is proposed to complement conventional binding studies of systems with two classes of binding constant with higher accuracy.

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The magnitude of the UV-spectral change of chlorpromazine increases in the presence of increasing concentrations of alcohols or fatty acids and with increasing chain length. A maximum is reached with 14.0- or 16.0-fatty acids. The differential spectrum is still larger with unsaturated fatty acids, a maximum effect being obtained with one cis-double bond. The spectral change is abolished by chaotropic and enhanced by antichaotropic agents.

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