Novel NIR-II organic fluorophores were designed and synthesized using an AIE and highly twisted donor–acceptor distortion strategy for bio-imaging beyond 1550 nm.
The first small-molecule based αvβ3-targeted NIR-II/PET dual-modal probes via base-catalyzed thiol-addition chemistry were concisely assembled and evaluated.
The development of novel biodegradable and nontoxic fluorophores that integrate diagnosis and therapy for effective cancer treatment has obtained tremendous attention in the past decades. In this report, water-soluble and biocompatible small-molecule near-infrared II (NIR-II) fluorescent dye H2a-4T complexed with fetal bovine serum (FBS) and Cetuximab proteins with excellent optical properties and targeting ability is prepared. High spatial and temporal resolution imaging of hind limb vasculature and the lymphatic system of living mice using H2a-4T@FBS complex is demonstrated in precise NIR-II imaging-guided sentinel lymph node surgery. More importantly, H2a-4T@Cetuximab complex not only exhibits a remarkable cell-killing ability but also achieves highly active tumor targeting efficiency for epidermal growth factor receptor, overexpressing colorectal cancer which is beneficial to in vivo NIR-II fluorescent imaging-guided photothermal therapy of colon tumors. To the best of our knowledge, it is the first time that the concept of light-harvesting complex is exploited for enhancing the NIR-II signals and photothermal energy conversion in molecule-protein complex theranostic agent, making them a promising candidate for future clinical applications in cancer theranostics.
Highlights d Lack of K11 ubiquitin chains affects transcription of methionine pathway enzymes d A K48 ubiquitin chain on the transcription factor Met4 prevents mediator binding d Met4 activation is initiated by a change from K48 to K11 linkages on Met4 d K11 linkages do not compete with mediator binding and allow transcription
Traditional luminescent materials including fluorescent probes suffer from notorious aggregation‐caused quenching in aqueous solutions. Although several approaches such as the aggregation‐induced emission effect have been developed, it remains a significant challenge to identify an effective and efficient strategy to resolve this issue. Herein, quaternary ammonium salts Q8PBn and Q8PNap as a novel class of bright near infrared window II (NIR‐II, 1000–1700 nm) probes are designed and synthesized, and the twisted intramolecular charge transfer formation at the excited state can be effectively suppressed for the newly designed probes. Furthermore, Q8PNap complexation with fetal bovine serum (Q8PNap/FBS) significantly increases the quantum yield by ≈32‐fold compared with PEGylated tertiary amine Q8P, and Q8PNap/FBS is successfully used to achieve high spatial and temporal resolution imaging of hind limb vasculature, lymphatic system, and small tumor metastasis, as well as precise NIR‐II imaging‐guided tumor and lymph node surgery in small animal models for the first time.
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