Silica/polydopamine/silver (SiO 2 /PD/Ag) nanoparticles (NPs) with a core-shell-satellite structure were fabricated and the mechanisms of their antibacterial activity were investigated. In this reported work, the results of the reactive oxygen species (ROS) assays, the deoxyribonucleic acid (DNA) damage assays and a cell morphology observation confirmed that Vibrio natriegens, a gram negative bacterium and Bacillus subtilis, a gram positive bacterium could be inhibited by the NPs. Gram negative bacteria exhibited more sensitivity towards the Ag NPs because these NPs were associated with penetration into the cytoplasm, with the subsequent local interaction of Ag with the cell components; thus, causing damages to the cells. SiO 2 /PD/Ag NPs produced ROS which caused damage to the DNA leading to the suppression of transcription as detected by a reporter gene assay. Furthermore, ROS induced membrane damage was determined by transmission electron microscopy. Thus, the mechanisms of antibacterial activity were interpreted more precisely by using the aforementioned experiments. The results revealed that the production of ROS and damage to the membrane were the two major mechanisms of the bactericidal action of SiO 2 /PD/Ag NPs; thus, these NPs could be employed as effective antifouling agents.
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