The aim of the study was to examine the effect of orbital implantation on peripheral blood melatonin and sex hormone levels in pediatric patients with congenital eyeball dysplasia. A total of 28 cases of pediatric patients with congenital eyeball dysplasia diagnosed in the Second Affiliated Hospital of Xi'an Jiaotong University from June 2014 to December 2014 were selected for the study. The patients included those that received orbital implantation, and the melatonin levels in the peripheral blood in patients before and after operation was observed. In addition, the sex hormone levels and T lymphocytes, plasma reactive oxygen species (ROS) and VEGF levels, urine 8-OHdG and 8-isoPGF2α levels in patients before and after treatment were detected, followed by statistical analysis. As a result, after 3 months of orbital implantation, the sex hormone levels in peripheral blood in child patients fluctuated significantly, and differences were not statistically significant (P>0.05). The peripheral blood T lymphocytes and ROS levels were significantly lower than those before treatment, and the differences were statistically significant (P<0.05). The correlation analysis revealed that the peripheral blood melatonin levels were negatively related to ROS levels; the correlation coefficient was rs = −0.481 and P<0.05. In conclusion, orbital implantation does not have significant impact on sex hormone levels in child patients with congenital eyeball dysplasia. The hydroxyapatite orbital implantation can achieve more satisfactory curative effects, and there are fewer postoperative complications. It does not affect the appearance of the eye, and therefore, it is suitable for patients with congenital eyeball dysplasia.
Human intestinal bacteria play important roles in the nutrition, development, immunity and even psychological diseases of the host, researches about such microorganisms is prevalent in recent years. New omics technologies are of great help for us to explore new functions and composition of intestinal microbiome, while studies about the molecular mechanisms of the symbiosis and reciprocity between these commensals and host are significant and too, and an important approach in the field is to conduct targeted gene editing for intestinal anaerobes and verify the function of genes by knocking out, replacing or inserting genes. Although the traditional bacterial gene editing technologies show high accuracy for DNA modification, they have low recombination efficiency and a polar mutation effect. This hinders the functional researches of intestinal bacteria. CRISPR/Cas9 technology, which emerged about 10 years ago, has advantages of wide universality, simple design and operation, and has been widely used in gene 10 马俊泽 等:CRISPR/Cas9 基因编辑技术在肠道厌氧菌研究中的应用 http://www.lifescitech.org manipulation of different organisms. This paper briefly introduces the technical principle of CRISPR/Cas9 and summarizes the current application of this technology in researches of intestinal anaerobic bacteria, a few aspects we are concerning and the trends for future development are analyzed as well.
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