Wilms' tumor gene WT1 is overexpressed in leukemia and various types of solid tumors and plays an important role in leukemogenesis and tumorigenesis. We tested apoptosis-inducing ability of short hairpin RNAs targeting exon 5 (shWTE5), exon10 (shWTE10) and 3'UTR (shWT3U) of the WT1 gene. Among the three WT1-shRNAs, since shWTE5 most effectively induced apoptosis, its ability as an apoptosis-inducing agent was intensively examined. shWTE5 induced mitochondrial damage and resultant apoptosis in five WT1-expressing solid cancer cells originated from gastric (AZ-521), lung (LU99B), ovarian (TYKnuCPr) cancers, fibrosarcoma (HT-1080) and glioblastoma (A172). Moreover, shWTE5 significantly enhanced apoptosis induced by chemotherapeutic agents, doxorubicin (DOX) and etoposide (ETP), or by death ligand TRAIL in all of the four solid tumor cells examined (HT-1080, LU99B, TYK and A172). Transduction of one each of WT1 isoforms with exon 5 [17AA(+)KTS(+) and 17AA(+)KTS(-)] prevented mitochondrial damage induced by ETP or TRAIL and inhibited apoptosis. These results showed that shWTE5 induced apoptosis through the suppression of the WT1 isoform with exon 5. Furthermore, shWTE5 increased expression of proapoptotic Bak and Bax proteins and decreased antiapoptotic Bcl-xL and Bcl-2 proteins in WT1expressing HT-1080 cells, indicating that WT1 isoforms with exon 5 might play an antiapoptotic role through regulation of Bcl-2 family genes in solid tumor cells. The results presented here demonstrated that WT1-shRNA targeting exon 5 should serve as a potent anti-cancer agent for various types of solid tumors.

Long non-coding RNAs (lncRNAs) are frequently dysregulated in a variety of human cancers. However, their biological roles in these cancers remain incompletely understood. In this study, we analyze the gene expression profiles of colon cancer tissues and identify a previously unannotated lncRNA, FLJ39051, that we term GSEC (G-quadruplex-forming sequence containing lncRNA), as a lncRNA that is upregulated in colorectal cancer. We further demonstrate that knockdown of GSEC results in the reduction of colon cancer cell motility. We also show that GSEC binds to the DEAH box polypeptide 36 (DHX36) RNA helicase via its G-quadruplex-forming sequence and inhibits DHX36 G-quadruplex unwinding activity. Moreover, knockdown of DHX36 restores the reduced migratory activity of colon cancer cells caused by GSEC knockdown. These results suggest that GSEC plays an important role in colon cancer cell migration by inhibiting the function of DHX36 via its G-quadruplex structure.

Previous research studies mostly focused on enhancing the security of radio frequency identification (RFID) protocols for various RFID applications that rely on a centralized database. However, blockchain technology is quickly emerging as a novel distributed and decentralized alternative that provides higher data protection, reliability, immutability, transparency, and lower management costs compared with a conventional centralized database. These properties make it extremely suitable for integration in a supply chain management system. In order to successfully fuse RFID and blockchain technologies together, a secure method of communication is required between the RFID tagged goods and the blockchain nodes. Therefore, this paper proposes a robust ultra-lightweight mutual authentication RFID protocol that works together with a decentralized database to create a secure blockchain-enabled supply chain management system. Detailed security analysis is performed to prove that the proposed protocol is secure from key disclosure, replay, manin-the-middle, de-synchronization, and tracking attacks. In addition to that, a formal analysis is conducted using Gong, Needham, and Yahalom logic and automated validation of internet security protocols and applications tool to verify the security of the proposed protocol. The protocol is proven to be efficient with respect to storage, computational, and communication costs. In addition to that, a further step is taken to ensure the robustness of the protocol by analyzing the probability of data collision written to the blockchain. INDEX TERMS Blockchain, distributed ledger technology, radio frequency identification.

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High temperatures during rice grain ripening reduced yield and grain quality. The proportion of milky white grains was 43.6 % at 30°C but only 6.5 % at 25°C. Grain filling was initially faster at 30°C and finished earlier, and the final dry matter content was less, than at 25°C. High temperature strongly suppressed the expression of the sucrose transporter gene OsSUT1 and starch synthesisrelated genes SuSy2, AGPS2b, BEIIb and Granule-bound starch synthase in grains during early grain filling; the transcription levels of OsSUT1 at 14 days after flowering (DAF) were about 60 % lower in grains, flag leaf blade, flag leaf sheath and first leaf sheath. These facts are possibly involved in the earlier termination of grain filling at 21 DAF, following the rapid rise of grain dry weight from 0 to 7 DAF, due to possible reduction in assimilate supply via OsSUT1 under the high temperature. When panicles were partly clipped, the resultant increase in assimilate supply to the remaining grains significantly upregulated the expression of OsSUT1 and the starch synthesis-related genes at 14 DAF, which consequently accelerated starch accumulation in the grains and ultimately increased the grain weight of remaining grains at 30°C. These results indicate that high temperature during grain filling reduces grain yield and quality by changing the expression of OsSUT1 and starch synthase-related genes, resulting in earlier ripening due to hastened or premature assimilate supply to grains.