Using Alucian Blue 8GX (AB), a simple fractional determination method was developed for sodium chondroitin sulfate (ChS-Na) and hydroxypropylmethylcellulose (HPMC) respectively in a drug. From a sample solution containing both ChS-Na and HPMC, ChS-Na was precipitated selectively as the ChS-Na-AB complex under a low-pH condition. After dissolution of the ChS-Na-AB complex in an alkali solution, ChS-Na was determined by the absorbance at 615 nm. The supernatant of the sample solution was subjected to gel permeation chromatography (GPC). The amount of HPMC was determined by the peak height of HPMC. These methods have safty, high-specificity and high-repeatability. These methods are therefore useful for drugs which contain both acidic mucopolysaccharide and a non-ionic polymer.
Non-steroidal anti-in‰ammatory drugs (NSAIDs) are widely known as painkillers. The analgesic action of NSAIDs is attributable to the inhibition of prostaglandin synthesis that occurs in response to blocking cyclooxygenase activity. The eŠective dose of NSAIDs can vary depending on pain intensity and administration timing; however, there are few studies on this. This study aimed to elucidate whether the analgesic eŠect of NSAIDs changes depending on the situation in which they are taken and we focused on the NSAID, aspirin (ASP). In a rat model of brewer's yeast-induced in‰ammation, pain caused by 20% (w/v) brewer's yeast-treatment was deˆned as``strong pain'' and that caused by 2.5% (w/v) was deˆned as``weak pain''. The analgesic eŠect of ASP (low-dose; 44 mg/kg or high-dose; 66 mg/kg) against strong pain was dose-dependent, but that against weak pain was the same. Furthermore, we deˆned drug administration after 3 h of brewer's yeast-treatment as``late administration'' and that after 20 min as``early administration''. In the case of strong pain, the analgesic eŠect of``late ASP administration'' was dose-dependent, but that of``early ASP administration'' was the same. These results suggest that low-dose NSAIDs have an analgesic eŠect against weak pain or when taken early.
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