Background This study was designed to investigate the antitumor activity of the mAb (AC10364) in vitro and elucidate the related mechanisms of inhibition to cell growth using bel/fu cells treated with AC10364. Methods The inhibitory effects of AC10364 on the proliferation of Bel/fu cells were examined using a cytotoxicity assay. Apoptosis of Bel/fu cells was detected using FITC annexin V and PI staining following treatment with AC10364 for 24 h. The factors regulating apoptosis were identified by Western blot using lysates of Bel/fu cells treated with AC10364 for 0, 12, 24, or 36 h. Genes associated with tumorigenesis or growth were analyzed by reverse transcription–quantitative polymerase chain reaction using Bel/fu cells treated for 12, 24, or 36 h with AC10364. Results The early apoptotic ratios of Bel/fu cells treated with AC10364 increased in a dose-dependent manner. The levels of caspases, including cleaved caspase-3, caspase-3 and caspase-9, were significantly high in Bel/fu cells treated with AC10364 ( P <0.001). Compared with untreated cells, those exposed to AC10364 had showed significant downregulation of the expression of binding protein gene (G protein subunit α 15, GNA15) and other protein-coding genes, including fms-related tyrosine kinase 1(FLT1), nicotinamide phosphoribosyltransferase (NAMPT), netrin 4 (NTN4), platelet-derived growth factor subunit A (PDGFA), S100 calcium binding protein A11 (S100A11), tubulin β 3 class III (TUBB3), aldo-keto reductase family 1 member C3 (AKR1C3), endothelial PAS domain protein 1 (EPAS1), and interferon α-inducible protein 27 (IFI27) ( P <0.001). Two other genes, AXL receptor tyrosine kinase (AXL) and carboxypeptidase A4 (CPA4), were significantly upregulated ( P <0.001). Conclusion AC10364 inhibited cell viability and proliferation through aberrant expression of multiple genes associated with tumorigenesis or growth, which suggests that these genes may be promising therapeutic candidates for cancer therapy.
Ovarian cancer is the most common female malignancy and the main cause of death from gynecological malignancies. Resveratrol (3,4,5 tri-hydroxystilbene) is a phytoalexin and a polyphenolic compound present in human dietary material such as peanuts, mulberries, grapes and red wine. We demonstrated that resveratrol depressed the proliferation of HO8910PM cells in a dose-dependent and time-dependent manner in vitro (P < 0.001). Resveratrol not only decreased ovarian tumor volume and weight but also influence the tumor forming time in vivo (P < 0.001). The cell cycle S phase was arrested in response to resveratrol treatment. Caspases activity were also detected by western-blotting in HO8910PM cells treated with resveratrol. Caspase-3 or/and caspase-9 were highly activated in resveratrol-treated HO8910PM cells or xerngraft model (P < 0.001). Resveratrol significantly inhibits growth and induces apoptosis in HO8910PM cells both in vitro and in vivo, suggesting that resveratrol is a potential treatment or supplementary measure of ovarian carcinoma.
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