SUMMARY Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans. To date, however, whether human PSCs (hPSCs) can contribute to chimera formation in non-rodent species remains unknown. We systematically evaluate the chimeric competency of several types of hPSCs using a more diversified clade of mammals, the ungulates. We find that naïve hPSCs robustly engraft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implantation pig embryos. Instead, an intermediate hPSC type exhibits higher degree of chimerism and is able to generate differentiated progenies in post-implantation pig embryos.

We recognized that the envelope fluctuation and decay of seismic records carries ultra low-frequency (ULF, i.e., the frequency below the lowest frequency in the source spectrum) signals that can be used to estimate the long-wavelength velocity structure. We then developed envelope inversion for the recovery of low-wavenumber components of media (smooth background), so that the initial model dependence of waveform inversion can be reduced. We derived the misfit function and the corresponding gradient operator for envelope inversion. To understand the long-wavelength recovery by the envelope inversion, we developed a nonlinear seismic signal model, the modulation signal model, as the basis for retrieving the ULF data and studied the nonlinear scale separation by the envelope operator. To separate the envelope data from the wavefield data (envelope extraction), a demodulation operator (envelope operator) was applied to the waveform data. Numerical tests using synthetic data for the Marmousi model proved the validity and feasibility of the proposed approach. The final results of combined [Formula: see text] (envelope-inversion for smooth background plus waveform-inversion for high-resolution velocity structure) indicated that it can deliver much improved results compared with regular full-waveform inversion (FWI) alone. Furthermore, to test the independence of the envelope to the source frequency band, we used a low-cut source wavelet (cut from 5 Hz below) to generate the synthetic data. The envelope inversion and the combined [Formula: see text] showed no appreciable difference from the full-band source results. The proposed envelope inversion is also an efficient method with very little extra work compared with conventional FWI.

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