Endosulfan degrading ability of(dry weight) degraded 22.18 ug ml -1 endosulfan within 5 days at pH 7.0, 30℃ in batch shake flask cultures. Also, we an experimented recycle packed bed column mode and continuous packed bed column mode for endosulfan degradation. Under optimum operation condition, the immobilized cells in a laboratory scale pack bed column with support beads were able to degrade endosulfan completely in defined minimal salt medium at a maximum rate of 129.6 ug ml -1 per day. Moreover, the endosulfan degradation activity could be demonstrated at 4℃ for one month without significant decrease in activity. Results of this study suggest that immobilized cells of Klebsiella oxytoca KE-8 might be applicable to endosulfan contaminated site.
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