Objectives : This study was aimed to determine which pharmacopuncture was effective in relieving pain in standard pain model, acute grade 3 ankle sprain rat model. Methods : Percentage changes of weight bearing ratio(WBR), as pain indicator, were measured after anti-inflammation, neutro Eohyul, Hwangryunhaedock-decoction pharmacopuncture were injected to Guheo (GB40). Results : In three kinds of pharmacopuncture, neutro Eohyul and Hwangryunhaedock-decoction pharmacopuncture represented more effect in percentage changes of WBR than surgical(non treatment), needling group. Conclusions : Comparing of pharmacopuncture in grade 3 ankle sprain rat model, these results provide basis to select pharmacopuncture in sprain disease. Furthermore, mechanism of pharmacopuncture's analgesia, anti-inflammation should be progressed.
Purpose : The first purpose of this study is to find out whether the water extract of Rehmanniae Radix Preparat(RRP), Cuscutae Semen(CS) and their combination(Ssangbohwan, SBH) have the effect of suppressing Receptor activator of nuclear factor kappa-B ligand(RANKL)-induced osteoclast differentiation. The second purpose of this study is to find out whether the water extract of RRP, CS and SBH have the effect of inhibiting osteoporosis in an osteoporosis model induced by lipopolysaccharide(LPS). Methods : After promoting differentiation of osteoclasts by treating the RANKL, we observed the effect by the administration of RRP, CS and SBH. In addition, by means of Reverse transcription polymerase chain reaction(RT-PCR), we assayed mRNA expression levels of NFATc1, c-Fos, TRAP and GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, spermatogeni) from bone marrow macrophages(BMMs). Similarly, the protein expression levels of NFATc1 (Nuclear factor of activated T-cells, cytoplasmic1), C-Fos, MAPKs(Mitogen-activated protein kinases) and β-actin in cell lysates were analyzed by means of Western Blotting. Finally, we determined the anti-osteoporotic effects of RRP, CS and SBH, through the use of Lipopolysaccharide-induced bone-loss mouse. Results : RRP, CS and SBH showed remarkable inhibitive effect on RANKL-treated osteoclast differentiation without cytotoxicity. SBH inhibited the phosphorylation of p38, Jun N-terminal kinases(JNK), and I-κB and down-regulated the induction of c-Fos and NFATc1 by RANKL. RRP, CS suppressed degradation of I-κB, but it did not affect c-Fos and NFATc1 by RANKL. Lastly, in vivo data showed that RRP and SBH prevented bone erosion by LPS treatment. Conclusions : These results demonstrate SBH can be effective remedy for bone-loss diseases such as osteoporosis.
[Abstract]Objectives : ThepurposeofthisstudywastoevaluatetheeffectsofwaterextractsofEucommiae cortex(EC),Psoraleaesemen(PS),andtheircombinationonreceptoractivatorofnuclearfactor-kappa-Bligand(RANKL)-inducedosteoclastdifferentiation. Methods : WeassayedtheproteinexpressionlevelsofnuclearfactorofactivatedT-cells,cytoplasmic1(NFATc1),c-Fos,mitogen-activatedproteinkinases(MAPKs),andβ-actinincell lysatesusingwesternblotting.Similarly,mRNAexpressionlevelsofNFATc1,c-Fos,tartrateresistantacidphosphate(TRAP),andglyceraldehyde-3-phosphatedehydrogenase,spermatogeni(GAPDHS)frombonemarrowmacrophages(BMMs)wereanalyzedusingreverse transcription-polymerasechainreaction(RT-PCR).Furthermore,wedeterminedtheanti-osteoporoticeffectsofthewaterextractsofEC,PS,andtheircombinationinalipopolysaccharide (LPS)-inducedbone-lossmousemodel. Results : Theinvitro datarevealedshowedthatthecombinationofECandPSextractshowed amoreremarkableinhibitionofosteoclastdifferentiationthaneachherbdidalone.Thecombinationdownregulatedtheinductionofc-Fos,NFATc1,andTRAPbysuppressingthephosphorylation of p38 and c-Jun N-terminal kinases (JNKs) and inhibiting nuclear factor kappa-light-chain-enhancerofactivatedBcells(NF-κB).Lastly,theinvivo datashowed thatPSreducedtheLPS-inducedboneerosion. Conclusion : TheresultofthisstudysuggeststhatECandPScouldbepotentialtherapeutic agentsforbonelossdiseasessuchasosteoporosis.※ ThisstudywassupportedbyacademicresearchgrantofWonkwangUniversityin2017
Objectives : The purpose of this study is to report a case of Brown-Sequard syndrome caused by traumatic cervical fracture, presenting pain in the right upper extremity and back of the left hand, motor weakness in the right side and diminished pain and temperature in the left side. Methods : A patient received Korean medical treatment(acupuncture, electroacupuncture, herbal medicine, cupping, moxibustion, Silver Spike point electrotherapy(SSP)) and rehabilitation treatment. We evaluated pain with the Numeric Rating Scale(NRS), motor grade with a Medical Research Council(MRC) scale, sensory function and Modified Barthel Index(MBI). Results : After treatment, the patient showed considerable improvement in NRS, motor & sensory function, and MBI. Conclusions : Korean medical and rehabilitation treatments could be effective for Brown-Sequard syndrome patients. More extensive studies should be carried out.
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