Intractable skin defects, which involve excessive inflammation and bacterial infections, caused by burns, trauma, and diabetes are a major challenge for clinicians. Compared with traditional skin transplantation, tissue-engineered skin has the advantages of a wide range of sources, prominent biological activity, and no damage to the donor area during the operation. Therefore, an effective wound-healing mat with antibacterial, anti-inflammatory, and microvascularization bioactivities is urgent to be developed. In this study, we have synthesized a poly(ester-urethane)urea/silk fibroin/magnolol nanofibrous composite mat (PSM) through electrospinning and post-hydrogen bond cross-linking. The results show that the hybrid magnolol has no adverse effect on the microstructure, porosity, wettability, and mechanical properties of PSM. Antibacterial experiments and cytocompatibility in vitro have proved that the addition of magnolol significantly improves the antibacterial ability and promotes cell adhesion and proliferation of PSM. In addition, the wound model of rat back and H&E staining, Masson trichrome staining, and CD31 and CD68 immunofluorescence staining were performed for evaluating the therapeutic efficiency of PSM. All the results show that the better wound treatment effect of magnolol hybrid nanofibrous mats in infectious skin tissue defected repair indicates their great potential for wound healing clinically.
Due to the special three-dimensional gradient structure from the bone to tendon at the tendon-bone interface (TBI), it is difficult to achieve a good therapeutic effect after rotator cuff injury. Biomimetic tissue-engineered gradient biphasic fiber patches may be a potential scaffold for repair. In this study, a gradient bipolar nanofiber scaffold with a structure of biomimetic TBI (SPGM) was prepared by continuous layer-by-layer electrospinning using poly(glycolide-co-ε-caprolactone), silk fibroin, and mesoporous bioactive glasses. The physicochemical characterization and mechanical test results confirmed that the SPGM could effectively avoid the stress concentration caused by easy to aggregate components and exhibited significantly matched mechanical properties. Moreover, SPGM also exhibited higher porosity, larger specific surface area, and suitable pore size for the growth of host cells. Cytocompatibility experiments, immunofluorescence staining, and cell morphology further demonstrated that the bipolar structure of gradient patches could induce rapid proliferation and adhesion of fibroblasts and osteoblasts, respectively. Therefore, this gradient bipolar nanofiber scaffold maybe a reliable candidate for rotator cuff repair.
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