Oligosaccharides and sucrose are very important nutritional components in soybean seeds. However, little information is available about their inheritance. We used molecular markers to identify the genomic regions significantly associated with the quantitative trait locus (QTL) that controls oligosaccharide and sucrose contents in segregating F2.10 RI lines. Two related, but independent, QTLs were identified for oligosaccharides --near marker satt546 on linkage group (LG) Dlb+W and satt278 on LG L. Four others, for sucrose content, were located at LG B1 (satt197), Dlb+W (satt546), and L (satt523 and satt278). Finally, we found two common QTLs, on LG Dlb+W and L, that are associated with both oligosaccharides and sucrose.