Studies to exploit wild mushrooms as a source of biologically active compounds are gaining importance in the recent years. In that view, the present study was carried out to evaluate the bioactive compounds of six wild edible mushrooms: Lentinus squarrosulus Mont., Auricularia auricular-judae (Bull.) Wettst., Mycetinis copelandii (Desjardin) A.W. Wilson & Desjardin, Baeospora myosura (Fr.) Singer, Pleurotus ostreatus (Jacq. ex. fr) Kummer and Volvariella volvacea (Bull. ex. Fr.) Singer. The specimen was subjected to phytochemical screening using gas chromatography mass spectrometer (GCMS) with the view to identify the important volatile constituents. The dried sporocarp was subjected to methanol extraction using a Soxhlet extractor and rotatory evaporator. This led to the identification of 14, 26, 33, 5, 49 and 32 different compounds in L. squarrosulus, A. auricular-judae, M. copelandii, B. myosura, P. ostreatus and V. volvacea respectively. n-Hexadecanoic acid; 9,12-Octadecadienoic acid (Z,Z)- and 2(3H)-Furanone where the most frequently occurring compounds across the studied mushrooms. The identified compound reportedly shows diverse bioactive activities, including antiviral, anticancer, antimicrobial, antioxidant, hypocholesterolemic, anaphylactic, narcotic, neurostimulant, emollient, expectorant, laxative, pesticidal, insecticidal and insectifugal activities. This data may provide baseline information about the bioactive constituents of these species from Nigeria, in providing valuable compounds of substantial medicinal and agricultural importance.
Inedible mushrooms are endowed with diverse nutritional and nutraceutical compounds. In this study, the amino acid profile, and phytochemical fingerprints of four selected wild inedible mushroom species from Oban Division of Cross River National Park (CRNP), Nigeria including Crepidotus applanatus (Pers.) P. Kumm, Daldinia concentrica (Bolton) Cesati & de Notaris, Oxyporus populinus (Schumach.) Donk and Trametes versicolor (L.) Lloyd were investigated. The fresh sporocarps of study mushroom were collected from the decaying wood during July 2018. Samples for amino acids profiling were dried to constant weight, defatted, hydrolyzed, evaporated in a rotary evaporator and loaded into the cartridge of the Applied Biosystems PTH Amino Acid Analyzer and analyzed for 45 minutes. The samples for phytochemical analysis were subjected to methanol extraction using a Soxhlet extractor and rotatory evaporator and injected into the column of an Agilent gas chromatography mass spectrometer (GCMS). The results of amino acid analysis revealed the presence of ten essential and eight non-essential amino acids in varying quantities across the species. The total amino acid content was highest in C. applanatus followed by D. concentrica, T. versicolor, and O. populinus in decreasing order. Glutamic acid, Arginine, and Aspartic acid were present in the highest amounts while Cystine, Lysine, and Histidine were the least across the species. The phytochemical screening of the samples led to the identification of 24 different compounds in D. concentrica, six compounds each in C. applanatus and O. populinus and three compounds in T. versicolor. In terms of percentage composition, 4H-Imidazo(1,5-a)(1,4)benzodiazepine-3-carboxylic acid, 5,6-dihydro-5-methyl-6-oxo-, ethyl ester, 1-Methylbicyclo[3.2.1]octane, Acetamide, N-(6-acetylaminobenzothiazol-2-yl)-2-(adamantan-1-yl)- and 9,19-Cyclolanost-24-en-3-ol, (3.beta.)-,TMS derivative were the most dominant compounds in C. applanatus, D. concentrica and T. versicolor respectively. The identified compound reportedly shows diverse bioactive activities, including antiviral, anticancer, antioxidant, hypocholesterolemic, anaphylactic, neurostimulant, pesticidal, insecticide and insecticidal activities. This data may represent the baseline information on the amino acid profiles and bioactive constituents of inedible mushroom species from Nigeria, as sources of noble compounds of substantial nutritional, medicinal and agricultural importance.
Pleurotus ostreatus, an edible mushroom is an essential food product. Recognised as one of the cheapest sources of proteins, their high nutritional and anti-nutritional properties are of immense importance to humans. The present study evaluated the effect of sawdust and dried plantain leaves on the growth and yield of Pleurotus ostreatus. Sawdust and dried plantain leaves were composted into seven substrates; as single substrates and at different combinations. The composted substrates include sawdust 100 % (M0), Dry plantain leaves 100% (M1), Dry plantain leaves + sawdust at the ratio of 1:4 (M2), 2:3 (M3), 3:2 (M4), 1:1 (M5) and 4:1(M6). Growth parameters of interest assessed flush include mycelia running rate (MRR), area of pileus, length of the stipe, the girth of the stipe, fresh weight and dry weight, number of fruit body, total yield and biological efficiency. At the end of the three weeks spawn running period, M0 was overall best in supporting mycelia running rate with a mean MRR of 16.00 cm. M1 substrates produced mushroom with longer and bigger stipes (7.17 cm) at the first flush. A higher number of fruit bodies (82.66), total yield (130.35 g), and biological efficiency (43.45 g) were all seen in M0 substrates. Sawdust at 100 % (M0) proved to be the best substrate for the cultivation of Pleurotus ostreatus.
Aims: To investigate the growth response of Pleurotus ostreatus, a wood-rotting fungus, to different growth substrates [Sawdust (SD), dry banana leaves (BL) and a combination of both BL and SD (BLSD)] amended with waste [salon effluent (SE) and spent calcium-carbide (SC)]. Place and duration of study: Department of Plant and Ecological Studies, University of Calabar, Cross River State, Nigeria, between May 2015 and August 2015. Methodology: Amendments were applied to growth substrates at different levels of concentration as follows: 0 ml and 0 g, 5 ml and 5 g, 10 ml and 10 g, 15 ml and 15 g per kg substrate. Mature mushrooms were harvested and assessed on the following parameters; number of fruit bodies, fresh weight, dry weight, length of stipe, girth of stipe, pileus area using conventional method. Results: Number of fruitbodies, fresh weight, dry weight and stipe length increased with increase in concentration of additives. Best performances of these growth parameters were obtained at 15 g/kg and 15 ml/kg concentration. The highest number of fruitbodies (with a peak mean value of 28.42 fruitbodies at 15 g/kg concentration), highest value of fresh weight and dry weight were observed in SD. The longest stipe length, largest stipe girth and pileus area were observed in BLSD, though it exhibited least performances in other growth parameters. BLSD amended with salon effluent produced mushrooms with the largest pileus area (with a peak mean value of 53.8 cm2 at 15ml concentration) compared to the other substrates. Conclusion: This study reveals that all growth parameters of P. ostreatus assessed were positively influenced by all the levels of amendments on the substrates used in this study. Therefore, these wastes could be used to increase the yield of P. ostreatus and possibly remediate sites polluted by these wastes.
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