No abstract

Summary 1.Roads provide suitable conditions for the establishment and growth of exotic species. Most roads are bordered by drainage ditches forming a network of linear wetlands. Drainage ditches may serve as habitats and corridors facilitating the spread of aquatic invaders into the intersected ecosystems. The common reed Phragmites australis is one of these aquatic invaders frequently found in marshes and drainage ditches along roads. We hypothesized that highways have acted as corridors for the dispersal of the common reed and have contributed to the invasion of North American wetlands by this species. 2. We mapped the spatial distribution of the common reed along the highway network of the province of Quebec, Canada, where a large-scale invasion of this plant species has been reported since the 1960s. We also identified the genotype of common reed colonies using molecular tools and the main characteristics that favour the presence of the common reed in road ditches.3. Approximately 67% of the 1359 1-km highway sections surveyed during summer 2003 in Quebec had at least one common reed colony. End to end, common reed colonies totalled 324 km, i.e. 24% of the 1359 km surveyed. 4. Common reed colonies located along the highways were largely (99%) dominated by the exotic (Eurasian) genotype (haplotype M). 5. The common reed was more abundant along highways located in warm regions, with a sum of growing degree-days ( > 5 ° C, 12-month period) ≥ 1885, along highways built before the 1970s and in agricultural regions dominated by corn and soybean crops. Common reed colonies were larger when located along highways that were wide, built before the 1970s or in warm regions. This was particularly apparent if the roadside was bordered by a wetland. On the other hand, common reed colonies were more likely to be narrow when located near a woodland. 6. Synthesis and applications. Several disturbances (de-icing salts, ditch digging and agricultural nitrogen input) favour the development of large common reed colonies along roads, some of them expanding out of roadsides, particularly in wetlands. Reducing disturbances, leaving (or planting) a narrow (a few metres) hedge of trees or shrubs along highways or planting salt-resistant shrubs in roadside ditches could be efficient ways to slow the expansion of common reed or to confine the species to roadsides.

Polyploidy is known to be common in plants and recent work has focused on the rapid changes in genome structure and expression that occur upon polyploidization. In Arabidopsis, much of this work has been done on a synthetic allotetraploid obtained by crossing a tetraploid Arabidopsis thaliana (2n = 4x = 20) with A. arenosa (2n = 4x = 32). To explore an alternative route to polyploidy in this model species, we have developed a synthetic allopolyploid by crossing two diploid species: A. thaliana (2n = 2x = 10) and Arabidopsis lyrata subsp. petraea (2n = 2x = 16). F(1) hybrids were easy to obtain and phenotypically more similar to A. lyrata. Spontaneous chromosome doubling events occurred in about 25% of the F(1)s, thus restoring fertility. The resulting allotetraploids (2n = 26) exhibited many genomic changes typically reported upon polyploidization. Nucleolar dominance was observed as only the A. lyrata rDNA loci were expressed in the F(1) and allotetraploids. Changes in the degree of methylation were observed at almost 25% of the loci examined by MSAP analysis. Finally, structural genomic alterations did occur as a large deletion covering a significant portion of the upper arm of chromosome II was detected but no evidence of increased mobility of transposons was obtained. Such allotetraploids derived from two parents with sequenced (or soon to be sequenced) genomes offer much promise in elucidating the various changes that occur in newly synthesized polyploids.

The response of plants to herbivory usually varies with the grazing regime experienced. We investigated (i) if the timing and frequency of grazing affected plant growth, (ii) if faeces deposition by herbivores stimulated plant growth, and (iii) if grazing affected the total nonstructural carbohydrate (TNC) reserves in the below-ground vegetation of two arctic graminoids, Dupontiafisheri and Eriophorum scheuchzeri. 2 This study was conducted in polygon fens exposed to intense summer grazing by greater snow geese (Chen caerulescens atlantica) on Bylot Island (73?N) in the Canadian High Arctic. We manipulated the frequency (once or three times) and the timing (early, mid or late in the season) of grazing and faeces deposition in controlled grazing trials using captive goslings. 3 Although ungrazed plants were taller than grazed ones at the end of the season, data on cumulative tiller elongation (net above-ground height production) showed that plants grazed once or three times produced new foliage after each defoliation in both species. However, neither grazing (presence or absence) nor its frequency affected the net above-ground primary production (NAPP) or the number of tillers at the end of the summer. Nitrogen concentration was highest in plants grazed three times, intermediate in those grazed once, and lowest in ungrazed plants. 4 Timing of grazing and presence of goose faeces with or without grazing had no effect on plant growth. 5 Eriophorum plants grazed three times had less TNC in their below-ground tissues than ungrazed plants, and the trend was similar in Dupontia. 6 Dupontia and Eriophorum were able to compensate for leaves lost to grazing and to maintain production at a level similar to ungrazed plants, but at some cost (reduced below-ground reserves). The absence of an effect of faeces on plant growth may explain the absence of a positive effect of grazing on NAPP (i.e. overcompensation) in this ecosystem.

Arabidopsis lyrata, a close relative of the model plant Arabidopsis thaliana, is 1 of a few plant species for which the genome is to be entirely sequenced, which promises to yield important insights into genome evolution. Only 2 sparse linkage maps have been published, and these were based solely on markers derived from the A. thaliana genome. Because the genome of A. lyrata is practically twice as large as that of A. thaliana, the extent of map coverage of the A. lyrata genome remains uncertain. In this study, a 2-way pseudo-testcross strategy was used to construct genetic linkage maps of A. lyrata subsp. petraea and A. lyrata subsp. lyrata, using simple sequence repeat (SSR) and cleaved amplified polymorphic sequence (CAPS) markers from the A. thaliana genome, and anonymous amplified fragment length polymorphism (AFLP) markers that could potentially uncover regions unique to the A. lyrata genome. The SSR and CAPS markers largely confirmed the relationships between linkage groups in A. lyrata and A. thaliana. AFLP markers slightly increased the coverage of the A. lyrata maps, but mostly increased marker density on the linkage groups. We noted a much lower level of polymorphism and a greater segregation distortion in A. lyrata subsp. lyrata markers. The implications of these findings for the sequencing of the A. lyrata genome are discussed.